ArticleBiochemistry2013
Kinetic properties of an RNA enzyme that undergoes self-sustained exponential amplification.
Article in Biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Cross-chiral exponential amplification of an RNA enzyme.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Charge-density reduction promotes ribozyme activity in RNA-peptide coacervates via RNA fluidization and magnesium partitioning.Nature chemistry · 2022Article
- Lipid membranes modulate the activity of RNA through sequence-dependent interactions.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- New approach to assess sperm DNA fragmentation dynamics: Fine-tuning mathematical models.Journal of animal science and biotechnology · 2017Article
- Real-Time Detection of a Self-Replicating RNA Enzyme.Molecules (Basel, Switzerland) · 2016Article
- Thirty-five years of research into ribozymes and nucleic acid catalysis: where do we stand today?F1000Research · 2016Review
- When Competing Viruses Unify: Evolution, Conservation, and Plasticity of Genetic Identities.Journal of molecular evolution · 2015Article
- Ligand-dependent exponential amplification of self-replicating RNA enzymes.Methods in enzymology · 2015Article
- Highly efficient self-replicating RNA enzymes.Chemistry & biology · 2014Article
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Authors and funding
2 authors.
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Abstract
A special class of biochemical reactions involves a set of enzymes that generate additional copies of themselves and transfer heritable information from parent to progeny molecules, thus providing the basis for genetics and Darwinian evolution. Such a process has been realized with a pair of self-replicating RNA enzymes that undergo exponential amplification at a constant temperature. Exponential growth requires that the rate of production of new enzymes be directly proportional to the existing concentration of enzymes, which is the case for this system and provides a doubling time of ~20 min. However, the catalytic rate of the underlying enzymes is ~100-fold faster than the observed rate of replication. As in biological replication, other aspects of the system limit the generation time, chiefly the propensity of the substrate molecules to form nonproductive complexes that limit their availability for replication. An analysis of this and other kinetic properties of the self-replicating RNA enzymes reveals how exponential amplification is achieved and how the rate of amplification might be increased.
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