ArticlePloS one2013
Dynamin-catalyzed membrane fission requires coordinated GTP hydrolysis.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Dynamin-like Proteins Combine Mechano-constriction and Membrane Remodeling to Enable Two-Step Mitochondrial Fission via a "Snap-through" Instability.Journal of the American Chemical Society · 2025Article
- Dynamin-dependent vesicle twist at the final stage of clathrin-mediated endocytosis.Nature cell biology · 2021Article
- Polymer-like Model to Study the Dynamics of Dynamin Filaments on Deformable Membrane Tubes.Biophysical journal · 2019Article
- Steric interference from intrinsically disordered regions controls dynamin-related protein 1 self-assembly during mitochondrial fission.Scientific reports · 2018Article
- VIPP1 Involved in Chloroplast Membrane Integrity Has GTPase Activity in Vitro.Plant physiology · 2018Article
- A noncanonical role for dynamin-1 in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells.PLoS biology · 2018Article
- Article
- Regulation of dynamin family proteins by post-translational modifications.Journal of biosciences · 2017Review
- Article
- Membrane fission by dynamin: what we know and what we need to know.The EMBO journal · 2016Review
- Dynamin recruitment and membrane scission at the neck of a clathrin-coated pit.Molecular biology of the cell · 2014Article
- A dynamin mutant defines a superconstricted prefission state.Cell reports · 2014Article
- Regulating dynamin dynamics during endocytosis.F1000prime reports · 2014Review
- Dynamin rings: not just for fission.Traffic (Copenhagen, Denmark) · 2013Review
- Dual role of BAR domain-containing proteins in regulating vesicle release catalyzed by the GTPase, dynamin-2.The Journal of biological chemistry · 2013Article
- Building a fission machine--structural insights into dynamin assembly and activation.Journal of cell science · 2013Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Dynamin is the most-studied membrane fission machinery and has served as a paradigm for studies of other fission GTPases; however, several critical questions regarding its function remain unresolved. In particular, because most dynamin GTPase domain mutants studied to date equally impair both basal and assembly-stimulated GTPase activities, it has been difficult to distinguish their respective roles in clathrin-mediated endocytosis (CME) or in dynamin catalyzed membrane fission. Here we compared a new dynamin mutant, Q40E, which is selectively impaired in assembly-stimulated GTPase activity with S45N, a GTP-binding mutant equally defective in both basal and assembly-stimulated GTPase activities. Both mutants potently inhibit CME and effectively recruit other endocytic accessory proteins to stalled coated pits. However, the Q40E mutant blocks at a later step than S45N, providing additional evidence that GTP binding and/or basal GTPase activities of dynamin are required throughout clathrin coated pit maturation. Importantly, using in vitro assays for assembly-stimulated GTPase activity and membrane fission, we find that the latter is much more potently inhibited by both dominant-negative mutants than the former. These studies establish that efficient fission from supported bilayers with excess membrane reservoir (SUPER) templates requires coordinated GTP hydrolysis across two rungs of an assembled dynamin collar.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.