ArticlePloS one2013
Molecular uptake of chitooligosaccharides through chitoporin from the marine bacterium Vibrio harveyi.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Structure-based classification of bacterial chitooligosaccharide-specific outer membrane porins (ChiPs).The Journal of biological chemistry · 2026Article
- Functional genomics of chitin degradation by Vibrio parahaemolyticus reveals finely integrated metabolic contributions to support environmental fitness.PLoS genetics · 2025Article
- Bacteria face trade-offs in the decomposition of complex biopolymers.PLoS computational biology · 2024Article
- Periplasmic chitooligosaccharide-binding protein requires a three-domain organization for substrate translocation.Scientific reports · 2023Article
- Article
- Genomic analysis and chitinase characterization ofFrontiers in microbiology · 2023Article
- The C2 entity of chitosugars is crucial in molecular selectivity of the Vibrio campbellii chitoporin.The Journal of biological chemistry · 2021Article
- Probing the physiological roles of the extracellular loops of chitoporin from Vibrio campbellii.Biophysical journal · 2021Article
- Structural basis of chitin utilization by a GH20 β-N-acetylglucosaminidase from Vibrio campbellii strain ATCC BAA-1116.Acta crystallographica. Section D, Structural biology · 2021Article
- Chitoporin from Serratia marcescens: recombinant expression, purification and crystallization.Acta crystallographica. Section F, Structural biology communications · 2020Article
- Regulation of Chitin-Dependent Growth and Natural Competence inMicroorganisms · 2020Article
- Single-channel properties, sugar specificity, and role of chitoporin in adaptive survival ofThe Journal of biological chemistry · 2020Article
- Chitin Heterodisaccharide, Released from Chitin by Chitinase and Chitin Oligosaccharide Deacetylase, Enhances the Chitin-Metabolizing Ability of Vibrio parahaemolyticus.Journal of bacteriology · 2019Article
- Structure and function of a novel periplasmic chitooligosaccharide-binding protein from marineThe Journal of biological chemistry · 2018Article
- Structural basis for chitin acquisition by marine Vibrio species.Nature communications · 2018Article
- Functional analysis of an unusual porin-like channel that imports chitin for alternative carbon metabolism inThe Journal of biological chemistry · 2017Article
- Chitinase Expression in Listeria monocytogenes Is Influenced byApplied and environmental microbiology · 2017Article
- Identification and Functional Characterization of a Novel OprD-like Chitin Uptake Channel in Non-chitinolytic Bacteria.The Journal of biological chemistry · 2016Article
- Azide anions inhibit GH-18 endochitinase and GH-20 Exo β-N-acetylglucosaminidase from the marine bacterium Vibrio harveyi.Journal of biochemistry · 2016Article
- Probing the Catalytic Mechanism of Vibrio harveyi GH20 β-N-Acetylglucosaminidase by Chemical Rescue.PloS one · 2016Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChitin is the most abundant biopolymer in marine ecosystems. However, there is no accumulation of chitin in the ocean-floor sediments, since marine bacteria Vibrios are mainly responsible for a rapid turnover of chitin biomaterials. The catabolic pathway of chitin by Vibrios is a multi-step process that involves chitin attachment and degradation, followed by chitooligosaccharide uptake across the bacterial membranes, and catabolism of the transport products to fructose-6-phosphate, acetate and NH(3). PRINCIPAL
findingsThis study reports the isolation of the gene corresponding to an outer membrane chitoporin from the genome of Vibrio harveyi. This porin, expressed in E. coli, (so called VhChiP) was found to be a SDS-resistant, heat-sensitive trimer. Immunoblotting using anti-ChiP polyclonal antibody confirmed the expression of the recombinant ChiP, as well as endogenous expression of the native protein in the V. harveyi cells. The specific function of VhChiP was investigated using planar lipid membrane reconstitution technique. VhChiP nicely inserted into artificial membranes and formed stable, trimeric channels with average single conductance of 1.8±0.13 nS. Single channel recordings at microsecond-time resolution resolved translocation of chitooligosaccharides, with the greatest rate being observed for chitohexaose. Liposome swelling assays showed no permeation of other oligosaccharides, including maltose, sucrose, maltopentaose, maltohexaose and raffinose, indicating that VhChiP is a highly-specific channel for chitooligosaccharides. CONCLUSION/SIGNIFICANCE: We provide the first evidence that chitoporin from V. harveyi is a chitooligosaccharide specific channel. The results obtained from this study help to establish the fundamental role of VhChiP in the chitin catabolic cascade as the molecular gateway that Vibrios employ for chitooligosaccharide uptake for energy production.
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