ArticlePloS one2013
A new strategy to produce a defensin: stable production of mutated NP-1 in nitrate reductase-deficient Chlorella ellipsoidea.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 49 citations in OpenAlex.
- Genetic Transformation of the Marine Oleaginous Microalga, Marinichlorella sp. NKG400014.Marine biotechnology (New York, N.Y.) · 2025Article
- Edible microalgae: potential candidate for developing edible vaccines.Vegetos (Bareilly, India) · 2023Review
- Establishment of a Genome Editing Tool Using CRISPR-Cas9 inInternational journal of molecular sciences · 2021Article
- Article
- Overexpression of the Transcription FactorFrontiers in bioengineering and biotechnology · 2021Article
- Advantages of Heterotrophic Microalgae as a Host for Phytochemicals Production.Frontiers in bioengineering and biotechnology · 2021Review
- Intraoperative single administration of neutrophil peptide 1 accelerates the early functional recovery of peripheral nerves after crush injury.Neural regeneration research · 2020Article
- Review
- Evaluation of Microalgae as Immunostimulants and Recombinant Vaccines for Diseases Prevention and Control in Aquaculture.Frontiers in bioengineering and biotechnology · 2020Review
- A mutated rabbit defensin NP-1 produced by Chlorella ellipsoidea can improve the growth performance of broiler chickens.Scientific reports · 2019Article
- Stable nuclear transformation of rhodophyte species Porphyridium purpureum: advanced molecular tools and an optimized method.Photosynthesis research · 2019Article
- Natural Antimicrobial Peptides as Inspiration for Design of a New Generation Antifungal Compounds.Journal of fungi (Basel, Switzerland) · 2017Review
- Perspectives for clinical use of engineered human host defense antimicrobial peptides.FEMS microbiology reviews · 2017Review
- The Potential for Microalgae as Bioreactors to Produce Pharmaceuticals.International journal of molecular sciences · 2016Review
- Effects of neutrophils peptide-1 transgenic Chlorella ellipsoidea on the gut microbiota of male Sprague-Dawley rats, as revealed by high-throughput 16S rRNA sequencing.World journal of microbiology & biotechnology · 2016Article
- Transgene Expression in Microalgae-From Tools to Applications.Frontiers in plant science · 2016Review
- Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses.Photosynthesis research · 2015Review
- Overexpression of the soybean transcription factor GmDof4 significantly enhances the lipid content of Chlorella ellipsoidea.Biotechnology for biofuels · 2014Article
- Article
- Protein N-glycosylation in eukaryotic microalgae and its impact on the production of nuclear expressed biopharmaceuticals.Frontiers in plant science · 2014Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Defensins are small cationic peptides that could be used as the potential substitute for antibiotics. However, there is no efficient method for producing defensins. In this study, we developed a new strategy to produce defensin in nitrate reductase (NR)-deficient C. ellipsoidea (nrm-4). We constructed a plant expression vector carrying mutated NP-1 gene (mNP-1), a mature α-defensin NP-1 gene from rabbit with an additional initiator codon in the 5'-terminus, in which the selection markers were NptII and NR genes. We transferred mNP-1 into nrm-4 using electroporation and obtained many transgenic lines with high efficiency under selection chemicals G418 and NaNO(3). The mNP-1 was characterized using N-terminal sequencing after being isolated from transgenic lines. Excitingly, mNP-1 was produced at high levels (approximately 11.42 mg/l) even after 15 generations of continuous fermentation. In addition, mNP-1 had strong activity against Escherichia coli at 5 µg/ml. This research developed a new method for producing defensins using genetic engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.