ArticlePloS one2013
The function of the conserved regulatory element within the second intron of the mammalian Csf1r locus.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Depletion and replacement of tissue-resident macrophages in mice with germ-line deletion of a conserved enhancer in the Csf1r locus.Development (Cambridge, England) · 2026Article
- Review
- Gene regulatory network analysis predicts cooperating transcription factor regulons required for FLT3-ITD+ AML growth.Cell reports · 2023Article
- CNS Border-Associated Macrophages: Ontogeny and Potential Implication in Disease.Current issues in molecular biology · 2023Review
- CNS macrophages differentially rely on an intronicDevelopment (Cambridge, England) · 2020Article
- The M-CSF receptor in osteoclasts and beyond.Experimental & molecular medicine · 2020Review
- Deletion of a Csf1r enhancer selectively impacts CSF1R expression and development of tissue macrophage populations.Nature communications · 2019Article
- The evolution of the macrophage-specific enhancer (Fms intronic regulatory element) within the CSF1R locus of vertebrates.Scientific reports · 2017Article
- A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.Development (Cambridge, England) · 2014Article
- Conservation in first introns is positively associated with the number of exons within genes and the presence of regulatory epigenetic signals.BMC genomics · 2014Article
- CSF-1 receptor signaling in myeloid cells.Cold Spring Harbor perspectives in biology · 2014Review
- Lentiviral vectors containing mouse Csf1r control elements direct macrophage-restricted expression in multiple species of birds and mammals.Molecular therapy. Methods & clinical development · 2014Article
- Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
The gene encoding the receptor for macrophage colony-stimulating factor (CSF-1R) is expressed exclusively in cells of the myeloid lineages as well as trophoblasts. A conserved element in the second intron, Fms-Intronic Regulatory Element (FIRE), is essential for macrophage-specific transcription of the gene. However, the molecular details of how FIRE activity is regulated and how it impacts the Csf1r promoter have not been characterised. Here we show that agents that down-modulate Csf1r mRNA transcription regulated promoter activity altered the occupancy of key FIRE cis-acting elements including RUNX1, AP1, and Sp1 binding sites. We demonstrate that FIRE acts as an anti-sense promoter in macrophages and reversal of FIRE orientation within its native context greatly reduced enhancer activity in macrophages. Mutation of transcription initiation sites within FIRE also reduced transcription. These results demonstrate that FIRE is an orientation-specific transcribed enhancer element.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.