ArticlePloS one2013
Wound induced tanscriptional regulation of benzylisoquinoline pathway and characterization of wound inducible PsWRKY transcription factor from Papaver somniferum.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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48 citing papers in PubMed, 97 citations in OpenAlex.
- Regulation of important natural products biosynthesis by WRKY transcription factors in plants.Journal of advanced research · 2025Review
- Network-based identification of hub transcription factors associated with benzylisoquinoline alkaloid biosynthesis inBiochemistry and biophysics reports · 2025Article
- Wounding and Phospholipase C Inhibition: Evaluation of the Alkaloid Profiling in Opium Poppy.Plants (Basel, Switzerland) · 2025Article
- Comprehensive characterization of the WRKY gene family and their potential roles in regulation phenylphenalenone biosynthesis inFrontiers in plant science · 2025Article
- Genomic and cell-specific regulation of benzylisoquinoline alkaloid biosynthesis in opium poppy.Journal of experimental botany · 2025Review
- Advances in the biosynthesis of naturally occurring benzylisoquinoline alkaloids.Frontiers in plant science · 2025Review
- The effects of green and chemically-synthesized copper oxide nanoparticles on the production and gene expression of morphinan alkaloids in Oriental poppy.Scientific reports · 2024Article
- Promising approaches for simultaneous enhancement of medicinally significant benzylisoquinoline alkaloids in opium poppy.Frontiers in plant science · 2024Review
- Histological, chemical and gene expression differences between western redcedar seedlings resistant and susceptible to cedar leaf blight.Frontiers in plant science · 2024Article
- A pervasive phosphorylation cascade modulation of plant transcription factors in response to abiotic stress.Planta · 2023Review
- Pistachio genomes provide insights into nut tree domestication and ZW sex chromosome evolution.Plant communications · 2023Article
- WRKY transcription factors: evolution, regulation, and functional diversity in plants.Protoplasma · 2023Review
- Plant salinity stress, sensing, and its mitigation through WRKY.Frontiers in plant science · 2023Review
- Editorial: Stress-mediated regulation of plant specialized metabolism.Frontiers in plant science · 2023Article
- Genome-wide analysis of the WRKY genes and their important roles during cold stress in white clover.PeerJ · 2023Article
- Genomic profiling of WRKY transcription factors and functional analysis of CcWRKY7, CcWRKY29, and CcWRKY32 related to protoberberine alkaloids biosynthesis inFrontiers in genetics · 2023Article
- The tissue-specific chromatin accessibility landscape ofFrontiers in genetics · 2023Article
- Interplay of transcription factors orchestrating the biosynthesis of plant alkaloids.3 Biotech · 2022Review
- Transcription Factors in Alkaloid Engineering.Biomolecules · 2021Review
- Genome-Wide Profiling ofFrontiers in plant science · 2021Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wounding is required to be made in the walls of the green seed pod of Opium poppy prior exudation of latex. To withstand this kind of trauma plants regulate expression of some metabolites through an induced transcript level. 167 unique wound-inducible ESTs were identified by a repetitive round of cDNA subtraction after 5 hours of wounding in Papaver somniferum seedlings. Further repetitive reverse northern analysis of these ESTs revealed 80 transcripts showing more than two fold induction, validated through semi-quantitative RT-PCR & real time expression analysis. One of the major classified categories among identified ESTs belonged to benzylisoquinoline transcripts. Tissue specific metabolite analysis of benzylisoquinoline alkaloids (BIAs) in response to wounding revealed increased accumulation of narcotine and papaverine. Promoter analysis of seven transcripts of BIAs pathway showed the presence of W-box cis-element with the consensus sequence of TGAC, which is the proposed binding site for WRKY type transcription factors. One of the Wound inducible 'WRKY' EST isolated from our subtracted library was made full-length and named as 'PsWRKY'. Bacterially expressed PsWRKY interacted with the W-box element having consensus sequence TTGACT/C present in the promoter region of BIAs biosynthetic pathway genes. PsWRKY further activated the TYDC promoter in yeast and transiently in tobacco BY2 cells. Preferential expression of PsWRKY in straw and capsule and its interaction with consensus W-box element present in BIAs pathway gene transcripts suggest its possible involvement in the wound induced regulation of BIAs pathway.
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