ArticlePloS one2013
Silencing of the host factor eIF(iso)4E gene confers plum pox virus resistance in plum.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 88 citations in OpenAlex.
- Targeted knockout of a host peroxisomal peptidase confers field resistance to maize lethal necrosis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Targeting ParPMC Susceptibility Hubs in Prunus: eEF1A as a Conserved Mediator of Plum Pox Virus Infection in Nicotiana benthamiana.Molecular plant pathology · 2026Article
- Molecular gatekeepers: eukaryotic translation factors decoding plant-virus dynamics for resistance engineering.Stress biology · 2026Review
- First genomic insights into the introgression of almond PPV-Marcus resistance into peach.Frontiers in plant science · 2026Article
- Enhancing papaya resistance to ringspot virus through CRISPR/Cas9-mediated gene editing of eFrontiers in plant science · 2026Article
- Edited eukaryotic translation initiation factors confer resistance against maize lethal necrosis.Plant biotechnology journal · 2024Article
- Machine Learning to Identify Critical Biomarker Profiles in New SARS-CoV-2 Variants.Microorganisms · 2024Article
- Cell Fractionation and the Identification of Host Proteins Involved in Plant-Virus Interactions.Pathogens (Basel, Switzerland) · 2024Review
- Allele mining of eukaryotic translation initiation factor genes in Prunus for the identification of new sources of resistance to sharka.Scientific reports · 2023Article
- Effect of Gene Silencing of Translation Initiation Factors eIF(iso)4G and eIF(iso)4E on Sour Cherry Rootstock Resistance to Sharka Disease.International journal of molecular sciences · 2022Article
- Spontaneous, Artificial, and Genome Editing-Mediated Mutations inInternational journal of molecular sciences · 2022Review
- Editing melon eIF4E associates with virus resistance and male sterility.Plant biotechnology journal · 2022Article
- Perspectives on plant virus diseases in a climate change scenario of elevated temperatures.Stress biology · 2022Review
- Genome-Wide Identification and Expression Analysis of eIF Family Genes fromPlants (Basel, Switzerland) · 2022Article
- Simultaneously induced mutations in eIF4E genes by CRISPR/Cas9 enhance PVY resistance in tobacco.Scientific reports · 2022Article
- Temporal expression of defence and susceptibility genes and tospovirus accumulation in capsicum chlorosis virus-infected capsicum.Archives of virology · 2022Article
- Rose Rosette Disease Resistance Loci Detected in Two Interconnected Tetraploid Garden Rose Populations.Frontiers in plant science · 2022Article
- DecipheringMetabolites · 2021Review
- Variability in the Viral Protein Linked to the Genome of Turnip Mosaic Virus Influences Interactions withThe plant pathology journal · 2021Article
- RNA-Based Technologies for Engineering Plant Virus Resistance.Plants (Basel, Switzerland) · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plum pox virus (PPV) causes the most economically-devastating viral disease in Prunus species. Unfortunately, few natural resistance genes are available for the control of PPV. Recessive resistance to some potyviruses is associated with mutations of eukaryotic translation initiation factor 4E (eIF4E) or its isoform eIF(iso)4E. In this study, we used an RNA silencing approach to manipulate the expression of eIF4E and eIF(iso)4E towards the development of PPV resistance in Prunus species. The eIF4E and eIF(iso)4E genes were cloned from plum (Prunus domestica L.). The sequence identity between plum eIF4E and eIF(iso)4E coding sequences is 60.4% at the nucleotide level and 52.1% at the amino acid level. Quantitative real-time RT-PCR analysis showed that these two genes have a similar expression pattern in different tissues. Transgenes allowing the production of hairpin RNAs of plum eIF4E or eIF(iso)4E were introduced into plum via Agrobacterium-mediated transformation. Gene expression analysis confirmed specific reduced expression of eIF4E or eIF(iso)4E in the transgenic lines and this was associated with the accumulation of siRNAs. Transgenic plants were challenged with PPV-D strain and resistance was evaluated by measuring the concentration of viral RNA. Eighty-two percent of the eIF(iso)4E silenced transgenic plants were resistant to PPV, while eIF4E silenced transgenic plants did not show PPV resistance. Physical interaction between PPV-VPg and plum eIF(iso)4E was confirmed. In contrast, no PPV-VPg/eIF4E interaction was observed. These results indicate that eIF(iso)4E is involved in PPV infection in plum, and that silencing of eIF(iso)4E expression can lead to PPV resistance in Prunus species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.