ArticlePLoS biology2013
Localized DNA demethylation at recombination intermediates during immunoglobulin heavy chain gene assembly.
Article in PLoS biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Clinical immunology in chromatinopathies: a scoping review.Frontiers in immunology · 2026Article
- Epigenetic Alterations in Inborn Errors of Immunity.Journal of clinical medicine · 2022Review
- Recombination may occur in the absence of transcription in the immunoglobulin heavy chain recombination centre.Nucleic acids research · 2020Article
- Alterations in chromatin at antigen receptor loci define lineage progression during B lymphopoiesis.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The histone methyltransferase Setd2 is indispensable for V(D)J recombination.Nature communications · 2019Article
- Developmental regulation of DNA cytosine methylation at the immunoglobulin heavy chain constant locus.PLoS genetics · 2019Article
- Misregulation of theFrontiers in immunology · 2018Article
- Epigenetic Control of B Cell Development and B-Cell-Related Immune Disorders.Clinical reviews in allergy & immunology · 2016Review
- Dissecting Epigenetic Dysregulation of Primary Antibody Deficiencies.Journal of clinical immunology · 2016Review
- Insertion of an imprinted insulator into the IgH locus reveals developmentally regulated, transcription-dependent control of V(D)J recombination.Molecular and cellular biology · 2015Article
- Independent recruitment of Igh alleles in V(D)J recombination.Nature communications · 2014Article
- Histone methylation and V(D)J recombination.International journal of hematology · 2014Review
- Epigenetic regulation of monoallelic rearrangement (allelic exclusion) of antigen receptor genes.Frontiers in immunology · 2014Review
- The Interplay between Chromatin and Transcription Factor Networks during B Cell Development: Who Pulls the Trigger First?Frontiers in immunology · 2014Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Multiple epigenetic marks have been proposed to contribute to the regulation of antigen receptor gene assembly via V(D)J recombination. Here we provide a comprehensive view of DNA methylation at the immunoglobulin heavy chain (IgH) gene locus prior to and during V(D)J recombination. DNA methylation did not correlate with the histone modification state on unrearranged alleles, indicating that these epigenetic marks were regulated independently. Instead, pockets of tissue-specific demethylation were restricted to DNase I hypersensitive sites within this locus. Though unrearranged diversity (D(H)) and joining (J(H)) gene segments were methylated, DJ(H) junctions created after the first recombination step were largely demethylated in pro-, pre-, and mature B cells. Junctional demethylation was highly localized, B-lineage-specific, and required an intact tissue-specific enhancer, Eμ. We propose that demethylation occurs after the first recombination step and may mark the junction for secondary recombination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.