ArticleJournal of experimental botany2013
Small RNA and degradome sequencing reveal complex miRNA regulation during cotton somatic embryogenesis.
Article in Journal of experimental botany, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
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Who cites it
108 citing papers in PubMed.
- Auxin-induced AsARF16 complex orchestrates lncRNA125175-mediated ceRNA networks to regulate garlic somatic embryogenesis.Horticulture research · 2026Article
- Molecular insights of somatic embryogenesis in coconut.Molecular biology reports · 2025Review
- Integrated analysis of microRNAs and lncRNAs expression profiles reveals regulatory modules during adventitious shoot induction in Moringa oleifera Lam.BMC plant biology · 2024Article
- miR156-SPL and miR169-NF-YA Modules Regulate the Induction of Somatic Embryogenesis in Arabidopsis via LEC- and Auxin-Related Pathways.International journal of molecular sciences · 2024Article
- Proteomic and Phosphoproteomic Analyses during Plant Regeneration Initiation in Cotton (Genes · 2024Article
- Machine Learning Analysis of the Impact of Silver Nitrate and Silver Nanoparticles on Wheat (Plants (Basel, Switzerland) · 2023Article
- Modulation of Plant MicroRNA Expression: Its Potential Usability in Wheat (Current genomics · 2023Review
- Uncovering the miRNA-mediated regulatory network involved in Ma bamboo (Horticulture research · 2023Article
- Epigenetic modifications and miRNAs determine the transition of somatic cells into somatic embryos.Plant cell reports · 2023Review
- Article
- Construction of ceRNA Networks at Different Stages of Somatic Embryogenesis in Garlic.International journal of molecular sciences · 2023Article
- Genetic and epigenetic modes of the regulation of somatic embryogenesis: a review.Biologia futura · 2022Review
- miR171 modulates induction of somatic embryogenesis in citrus callus.Plant cell reports · 2022Article
- Global DNA Methylation and mRNA-miRNA Variations Activated by Heat Shock Boost Early Microspore Embryogenesis in Cabbage (International journal of molecular sciences · 2022Article
- miRNA Profiling and Its Role in Multi-Omics Regulatory Networks Connected with Somaclonal Variation in Cucumber (International journal of molecular sciences · 2022Article
- miR172 RegulatesCells · 2022Article
- Regulatory non-coding RNAs: Emerging roles during plant cell reprogramming andFrontiers in plant science · 2022Review
- Integrated analysis of small RNAs, transcriptome and degradome sequencing reveal the drought stress network inFrontiers in plant science · 2022Article
- Dynamic Transcriptome Analysis Reveals Complex Regulatory Pathway Underlying Induction and Dose Effect by Different Exogenous Auxin IAA and 2,4-D DuringFrontiers in plant science · 2022Article
- Genome-wide identification and analysis of Oleosin gene family in four cotton species and its involvement in oil accumulation and germination.BMC plant biology · 2021Article
48 more citing papers are in PubMed but not listed here.
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are endogenous non-coding ~21 nucleotide RNAs that regulate gene expression at the transcriptional and post-transcriptional levels in plants and animals. They play an important role in development, abiotic stress, and pathogen responses. miRNAs with their targets have been widely studied in model plants, but limited knowledge is available on the small RNA population of cotton (Gossypium hirsutum)-an important economic crop, and global identification of related targets through degradome sequencing has not been developed previously. In this study, small RNAs and their targets were identified during cotton somatic embryogenesis (SE) through high-throughput small RNA and degradome sequencing, comparing seedling hypocotyl and embryogenic callus (EC) of G. hirsutum YZ1. A total of 36 known miRNA families were found to be differentially expressed, of which 19 miRNA families were represented by 29 precursors. Twenty-five novel miRNAs were identified. A total of 234 transcripts in EC and 322 transcripts in control (CK) were found to be the targets of 23 and 30 known miRNA families, respectively, and 16 transcripts were targeted by eight novel miRNAs. Interestingly, four trans-acting small interfering RNAs (tas3-siRNAs) were also found in degradome libraries, three of which perfectly matched their precursors. Several targets were further validated via RNA ligase-mediated rapid amplification of 5' cDNA ends (RLM 5'-RACE). The profiling of the miRNAs and their target genes provides new information on the miRNAs network during cotton SE.
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