ArticleThe American journal of tropical medicine and hygiene2013
The use of fluorescent fragment length analysis (PCR-FFL) in the direct diagnosis and identification of cutaneous Leishmania species.
Article in The American journal of tropical medicine and hygiene, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 11 citations in OpenAlex.
- Genetic diversity of Phlebotomus perniciosus populations between insular and mainland regions in the leishmaniasis-endemic western Mediterranean area.Parasites & vectors · 2026Article
- Underestimation of Human Cutaneous Leishmaniasis Caused byMicroorganisms · 2023Article
- Case Report: Diffuse Cutaneous Leishmaniasis byThe American journal of tropical medicine and hygiene · 2018Article
- Clinical and diagnostic aspects of feline cutaneous leishmaniosis in Venezuela.Parasites & vectors · 2018Article
- Real-time PCR using FRET technology for Old World cutaneous leishmaniasis species differentiation.Parasites & vectors · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leishmaniasis is a disease caused by different species belonging to the genus Leishmania. It presents different epidemiological and clinical features and requires the development of rapid, sensitive techniques to improve specific diagnosis. In this study, we compared the traditional technique of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with PCR-fluorescent fragment length analysis (PCR-FFL). Fluorescently tagged primers, designed in the rRNA fragment ITS-1 and 7SL region, were used to amplify fragments, which were later digested and whose sizes were accurately determined using an automated DNA sequencer. We validated the technique using 19 Leishmania strains from five cutaneous Leishmania species before testing 36 clinical samples: 23 skin biopsies and 13 skin scrapings/lesion exudates on filter paper. In real diagnostic, PCR-FFL has proved to be quick, accurate, and more sensitive (83.3% testing the ITS-1 fragment and 94.4% testing the 7SL) than PCR-RFLP analysis (75% and 80.6%). Filter papers improved the specific diagnosis in both techniques using non-invasive samples.
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