ArticleJournal of plant research2013
Microdissection and painting of the Y chromosome in spinach (Spinacia oleracea).
Article in Journal of plant research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Sex chromosome pairing and multivalent associations during meiosis in diploid and polyploid Silene latifolia.Plant physiology · 2026Article
- Article
- Identification of structural variation and polymorphisms of a sex co-segregating scaffold in spinach.Plant reproduction · 2022Article
- Cloning and physical localization of male-biased repetitive DNA sequences inComparative cytogenetics · 2021Article
- Construction of high-density genetic maps defined sex determination region of the Y chromosome in spinach.Molecular genetics and genomics : MGG · 2021Article
- Comparative transcriptome analysis of male and female flowers in Spinacia oleracea L.BMC genomics · 2020Article
- Genome-Wide Analysis of Transposable Elements and Satellite DNAs inFrontiers in plant science · 2020Article
- Genome-wide characterization of microsatellites and genetic diversity assessment of spinach in the Chinese germplasm collection.Breeding science · 2018Article
- Development of an X-specific marker and identification of YY individuals in spinach.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- Article
- Rapid cloning and bioinformatic analysis of spinach Y chromosome-specific EST sequences.Journal of genetics · 2015Article
- Sex determination in dioecious Mercurialis annua and its close diploid and polyploid relatives.Heredity · 2015Article
- Use of laser microdissection for the construction of Humulusjaponicus Siebold et Zuccarini, 1846 (Cannabaceae) sex chromosome-specific DNA library and cytogenetics analysis.Comparative cytogenetics · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spinach has long been used as a model for genetic and physiological studies of sex determination and expression. Although trisomic analysis from a cross between diploid and triploid plants identified the XY chromosome as the largest chromosome, no direct evidence has been provided to support this at the molecular level. In this study, the largest chromosomes of spinach from mitotic metaphase spreads were microdissected using glass needles. Degenerate oligonucleotide primed polymerase chain reaction was used to amplify the dissected chromosomes. The amplified products from the Y chromosome were identified using the male-specific marker T11A. For the first time, the largest spinach chromosome was confirmed to be a sex chromosome at the molecular level. PCR products from the isolated chromosomes were used in an in situ probe mixture for painting the Y chromosome. The fluorescence signals were mainly distributed on all chromosomes and four pair of weaker punctate fluorescence signal sites were observed on the terminal region of two pair of autosomes. These findings provide a foundation for the study of sex chromosome evolution in spinach.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.