ArticleBMC genomics2013
Analysis of the leaf transcriptome of Musa acuminata during interaction with Mycosphaerella musicola: gene assembly, annotation and marker development.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 55 citations in OpenAlex.
- Genetic Improvement for Resistance to Black Sigatoka in Bananas: A Systematic Review.Frontiers in plant science · 2021Pooled it
- Characterization of microRNAs and Target Genes inPlants (Basel, Switzerland) · 2023Article
- Genome-wide identification, characterization, and evolutionary analysis of NBS genes and their association with disease resistance in Musa spp.Functional & integrative genomics · 2022Article
- Transcriptome Profiling of the Resistance Response ofInternational journal of molecular sciences · 2022Article
- Infection by3 Biotech · 2022Article
- Gene Expression, Histology and Histochemistry in the Interaction betweenPlants (Basel, Switzerland) · 2022Article
- Genetic Characteristics and Metabolic Interactions betweenPlants (Basel, Switzerland) · 2022Review
- Article
- Deciphering the Omics of Plant-Microbe Interaction: Perspectives and New Insights.Current genomics · 2020Review
- Stable reference genes for RT-qPCR analysis of gene expression in the Musa acuminata-Pseudocercospora musae interaction.Scientific reports · 2019Article
- MusatransSSRDB (a transcriptome derived SSR database) - An advanced tool for banana improvement.Journal of biosciences · 2019Article
- Development, characterization, and cross-amplification of microsatellite markers forApplications in plant sciences · 2018Article
- Gene expression analysis in Musa acuminata during compatible interactions with Meloidogyne incognita.Annals of botany · 2017Article
- Transcriptome sequencing of Mycosphaerella fijiensis during association with Musa acuminata reveals candidate pathogenicity genes.BMC genomics · 2016Article
- Article
- Article
- De novo transcriptome sequence assembly from coconut leaves and seeds with a focus on factors involved in RNA-directed DNA methylation.G3 (Bethesda, Md.) · 2014Article
- Identification of glutathione S-transferase genes responding to pathogen infestation in Populus tomentosa.Functional & integrative genomics · 2014Article
- Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough banana (Musa sp.) is an important edible crop, contributing towards poverty alleviation and food security, limited transcriptome datasets are available for use in accelerated molecular-based breeding in this genus. 454 GS-FLX Titanium technology was employed to determine the sequence of gene transcripts in genotypes of Musa acuminata ssp. burmannicoides Calcutta 4 and M. acuminata subgroup Cavendish cv. Grande Naine, contrasting in resistance to the fungal pathogen Mycosphaerella musicola, causal organism of Sigatoka leaf spot disease. To enrich for transcripts under biotic stress responses, full length-enriched cDNA libraries were prepared from whole plant leaf materials, both uninfected and artificially challenged with pathogen conidiospores.
resultsThe study generated 846,762 high quality sequence reads, with an average length of 334 bp and totalling 283 Mbp. De novo assembly generated 36,384 and 35,269 unigene sequences for M. acuminata Calcutta 4 and Cavendish Grande Naine, respectively. A total of 64.4% of the unigenes were annotated through Basic Local Alignment Search Tool (BLAST) similarity analyses against public databases.Assembled sequences were functionally mapped to Gene Ontology (GO) terms, with unigene functions covering a diverse range of molecular functions, biological processes and cellular components. Genes from a number of defense-related pathways were observed in transcripts from each cDNA library. Over 99% of contig unigenes mapped to exon regions in the reference M. acuminata DH Pahang whole genome sequence. A total of 4068 genic-SSR loci were identified in Calcutta 4 and 4095 in Cavendish Grande Naine. A subset of 95 potential defense-related gene-derived simple sequence repeat (SSR) loci were validated for specific amplification and polymorphism across M. acuminata accessions. Fourteen loci were polymorphic, with alleles per polymorphic locus ranging from 3 to 8 and polymorphism information content ranging from 0.34 to 0.82.
conclusionsA large set of unigenes were characterized in this study for both M. acuminata Calcutta 4 and Cavendish Grande Naine, increasing the number of public domain Musa ESTs. This transcriptome is an invaluable resource for furthering our understanding of biological processes elicited during biotic stresses in Musa. Gene-based markers will facilitate molecular breeding strategies, forming the basis of genetic linkage mapping and analysis of quantitative trait loci.
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