Evidence map›Paper›PMID 23379639›Full record

ArticleStem cells and development2013

Epigenetic marks define the lineage and differentiation potential of two distinct neural crest-derived intermediate odontogenic progenitor populations.

Gokul Gopinathan, Antonia Kolokythas, Xianghong Luan, Thomas G H Diekwisch

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells and development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 73 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Gokul GopinathanUIC Brodie Laboratory for Craniofacial Genetics, UIC College of Dentistry, Chicago, Illinois 60612, USA.
Antonia Kolokythas
Xianghong Luan
Thomas G H Diekwisch
University of Illinois Chicago · USIllinois College · US

Funding

Small Molecule Microenvironment Design for Craniofacial Bone Regeneration and EngineeringR01DE019463 · NIDCR · UNIVERSITY OF ROCHESTER · PI LUAN, XIANGHONG · 2009 to 2021
$2.8M
NIDCR NIH HHS DE019463
6 · The paper itself

Abstract

Epigenetic mechanisms, such as histone modifications, play an active role in the differentiation and lineage commitment of mesenchymal stem cells. In the present study, epigenetic states and differentiation profiles of two odontogenic neural crest-derived intermediate progenitor populations were compared: dental pulp (DP) and dental follicle (DF). ChIP on chip assays revealed substantial H3K27me3-mediated repression of odontoblast lineage genes DSPP and dentin matrix protein 1 (DMP1) in DF cells, but not in DP cells. Mineralization inductive conditions caused steep increases of mineralization and patterning gene expression levels in DP cells when compared to DF cells. In contrast, mineralization induction resulted in a highly dynamic histone modification response in DF cells, while there was only a subdued effect in DP cells. Both DF and DP progenitors featured H3K4me3-active marks on the promoters of early mineralization genes RUNX2, MSX2, and DLX5, while OSX, IBSP, and BGLAP promoters were enriched for H3K9me3 or H3K27me3. Compared to DF cells, DP cells expressed higher levels of three pluripotency-associated genes, OCT4, NANOG, and SOX2. Finally, gene ontology comparison of bivalent marks unique for DP and DF cells highlighted cell-cell attachment genes in DP cells and neurogenesis genes in DF cells. In conclusion, the present study indicates that the DF intermediate odontogenic neural crest lineage is distinguished from its DP counterpart by epigenetic repression of DSPP and DMP1 genes and through dynamic histone enrichment responses to mineralization induction. Findings presented here highlight the crucial role of epigenetic regulatory mechanisms in the terminal differentiation of odontogenic neural crest lineages.

Indexed as

Epigenesis, GeneticAdolescentBiomarkersCalcification, PhysiologicCell DifferentiationCell LineageChildDental PulpDental SacDentin SialophosphoproteinExtracellular Matrix ProteinsGene Expression ProfilingGene Expression Regulation, DevelopmentalHumansJumonji Domain-Containing Histone DemethylasesMesenchymal Stem CellsBiomarkersDentin SialophosphoproteinDMP1 protein, humanExtracellular Matrix ProteinsJumonji Domain-Containing Histone DemethylasesKDM6B protein, humanPhosphoproteinsSialoglycoproteins

Identifiers

PMID23379639
PMCPMC3668503
OpenAlexW2013127012

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.