ArticleBMC plant biology2013
Single-cell-based system to monitor carrier driven cellular auxin homeostasis.
Article in BMC plant biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Integrated Transcriptomic and Metabolomic Analysis Reveals Biochar-Induced Enhancement of Growth and Secondary Metabolism in the Medicinal PlantInternational journal of molecular sciences · 2025Article
- Advances in Plant Auxin Biology: Synthesis, Metabolism, Signaling, Interaction with Other Hormones, and Roles under Abiotic Stress.Plants (Basel, Switzerland) · 2024Review
- Auxin- and pH-induced guttation in Phycomyces sporangiophores: relation between guttation and diminished elongation growth.Protoplasma · 2023Article
- Mutation ofInternational journal of molecular sciences · 2022Article
- Chemical Biology in Auxin Research.Cold Spring Harbor perspectives in biology · 2021Review
- KUP9 maintains root meristem activity by regulating KEMBO reports · 2020Article
- All Roads Lead to Auxin: Post-translational Regulation of Auxin Transport by Multiple Hormonal Pathways.Plant communications · 2020Review
- PIN-LIKES Coordinate Brassinosteroid Signaling with Nuclear Auxin Input in Arabidopsis thaliana.Current biology : CB · 2020Article
- Comparative Analysis of the PIN Auxin Transporter Gene Family in Different Plant Species: A Focus on Structural and Expression Profiling of PINs inInternational journal of molecular sciences · 2019Article
- PILS6 is a temperature-sensitive regulator of nuclear auxin input and organ growth inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Light triggers PILS-dependent reduction in nuclear auxin signalling for growth transition.Nature plants · 2017Article
- Regulation of polar auxin transport by protein and lipid kinases.Journal of experimental botany · 2016Review
- A 3-dimensional fibre scaffold as an investigative tool for studying the morphogenesis of isolated plant pells.BMC plant biology · 2015Article
- Brassinazole resistant 1 (BZR1)-dependent brassinosteroid signalling pathway leads to ectopic activation of quiescent cell division and suppresses columella stem cell differentiation.Journal of experimental botany · 2015Article
- ER network homeostasis is critical for plant endosome streaming and endocytosis.Cell discovery · 2015Article
- PIN-dependent auxin transport: action, regulation, and evolution.The Plant cell · 2015Review
- Auxin transport sites are visualized in planta using fluorescent auxin analogs.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Functional Analysis of the Hydrophilic Loop in Intracellular Trafficking of Arabidopsis PIN-FORMED Proteins.The Plant cell · 2014Article
- A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics.Scientific reports · 2013Article
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8 authors.
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Abstract
backgroundAbundance and distribution of the plant hormone auxin play important roles in plant development. Besides other metabolic processes, various auxin carriers control the cellular level of active auxin and, hence, are major regulators of cellular auxin homeostasis. Despite the developmental importance of auxin transporters, a simple medium-to-high throughput approach to assess carrier activities is still missing. Here we show that carrier driven depletion of cellular auxin correlates with reduced nuclear auxin signaling in tobacco Bright Yellow-2 (BY-2) cell cultures.
resultsWe developed an easy to use transient single-cell-based system to detect carrier activity. We use the relative changes in signaling output of the auxin responsive promoter element DR5 to indirectly visualize auxin carrier activity. The feasibility of the transient approach was demonstrated by pharmacological and genetic interference with auxin signaling and transport. As a proof of concept, we provide visual evidence that the prominent auxin transport proteins PIN-FORMED (PIN)2 and PIN5 regulate cellular auxin homeostasis at the plasma membrane and endoplasmic reticulum (ER), respectively. Our data suggest that PIN2 and PIN5 have different sensitivities to the auxin transport inhibitor 1-naphthylphthalamic acid (NPA). Also the putative PIN-LIKES (PILS) auxin carrier activity at the ER is insensitive to NPA in our system, indicating that NPA blocks intercellular, but not intracellular auxin transport.
conclusionsThis single-cell-based system is a useful tool by which the activity of putative auxin carriers, such as PINs, PILS and WALLS ARE THIN1 (WAT1), can be indirectly visualized in a medium-to-high throughput manner. Moreover, our single cell system might be useful to investigate also other hormonal signaling pathways, such as cytokinin.
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