ArticleScientific reports2013
A simple medium enables bovine embryos to be held for seven days at 4°C.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Development of an oscillating magnetic field-assisted supercooling system for bovine embryo preservation.Journal of animal science and technology · 2026Article
- Role of Glutathione in Alleviating Chilling Injury in Bovine Blastocysts: Mitochondrial Restoration and Apoptosis Inhibition.Antioxidants (Basel, Switzerland) · 2026Article
- Toll-like receptor 2 activation of early divided bovine embryo promotes its viability and development competence in vitro.Scientific reports · 2025Article
- Toll-like Receptor 2 is Involved in Calcium Influx and Acrosome Reaction to Facilitate Sperm Penetration to Oocytes DuringFrontiers in cell and developmental biology · 2022Article
- Mild hypothermia promotes the viability of in vitro-produced bovine blastocysts and their transcriptional expression of the cold-inducible transcription factor Rbm3 during in vitro culture.The Journal of reproduction and development · 2019Article
- Prevention of hatching of porcine morulae and blastocysts by liquid storage at 20 °C.Scientific reports · 2019Article
- Effect of a single epidural administration of follicle-stimulating hormone via caudal vertebrae on superstimulation for in vivo and in vitro embryo production in Japanese black cows.The Journal of reproduction and development · 2018Article
- Oviduct epithelium induces interferon-tau in bovine Day-4 embryos, which generates an anti-inflammatory response in immune cells.Scientific reports · 2018Article
- Holding of bovine blastocysts at suprazero temperatures using small molecules.Scientific reports · 2017Article
- Bovine embryo induces an anti-inflammatory response in uterine epithelial cells and immune cells in vitro: possible involvement of interferon tau as an intermediator.The Journal of reproduction and development · 2017Article
- Effect of medium additives during liquid storage on developmental competence of in vitro matured bovine oocytes.Animal science journal = Nihon chikusan Gakkaiho · 2017Article
- Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant.Marine drugs · 2017Review
- Effective vitrification and warming of porcine embryos using a pH-stable, chemically defined medium.Scientific reports · 2016Article
- Prolonging hypothermic storage (4 C) of bovine embryos with fish antifreeze protein.The Journal of reproduction and development · 2015Article
- Successful non-surgical deep uterine transfer of porcine morulae after 24 hour culture in a chemically defined medium.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryopreservation methods using liquid nitrogen (LN(2)) for gametes and embryos are prevalent in mammalian artificial reproduction. However, the pregnancy rate from frozen embryos has not improved over the past two decades because freeze-thawing causes significant damage. The strict regulation of transportation of LN(2) containers by airlines also limits exchange between breeders. In this article, we introduce a medium that enabled bovine embryos to be held for up to 7 days at 4°C. A pregnancy rate of 75% (24/32) was obtained for embryos held for 7 days in this medium and transferred to primed recipients. Its constituents were medium 199, foetal bovine serum, and HEPES for buffering. This technique will enable LN(2)-free storage and air transportation of embryos provided transplantation to recipients can be completed within 7 days.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.