Evidence map›Paper›PMID 23378573›Full record

ReviewMicrobiology (Reading, England)2013

An update on the molecular genetics toolbox for staphylococci.

Marcel Prax, Chia Y Lee, Ralph Bertram

Abstract readReview
In one paragraph

Review in Microbiology (Reading, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Journal of bacteriology · 2025
    Review
  2. Article
  3. Article
  4. Recombineering in Staphylococcus aureus.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Generation of a Stable Plasmid forApplied and environmental microbiology · 2016
    Article
  14. Article
  15. Article
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Marcel PraxDepartment of Microbial Genetics, Interfaculty Institute of Microbiology and Infection Medicine Tübingen (IMIT), Faculty of Science, University of Tübingen, Waldhäuser Str. 70/8, 72076 Tübingen, Germany.
Chia Y LeeDepartment of Microbiology and Immunology, University of Arkansas for Medical Sciences, 4301 W. Markham Street, Slot 511, Little Rock, AR 72205, USA.
Ralph BertramDepartment of Microbial Genetics, Interfaculty Institute of Microbiology and Infection Medicine Tübingen (IMIT), Faculty of Science, University of Tübingen, Waldhäuser Str. 70/8, 72076 Tübingen, Germany.

Funding

TYPE 8 CAPSULE OF STAPHYLOCOCCUS AUREUSR01AI037027 · NIAID · UNIV OF ARKANSAS FOR MED SCIS · PI LEE, CHIA Y. · 1995 to 2013
$3.2M
Regulation of Staphylococcus aureus biofilm by RbfR01AI067857 · NIAID · UNIV OF ARKANSAS FOR MED SCIS · PI LEE, CHIA Y. · 2007 to 2011
$1.8M
NIAID NIH HHS AI37027NIAID NIH HHS AI67857NIAID NIH HHS R01 AI037027NIAID NIH HHS R01 AI067857
6 · The paper itself

Abstract

Staphylococci are Gram-positive spherical bacteria of enormous clinical and biotechnological relevance. Staphylococcus aureus has been extensively studied as a model pathogen. A plethora of methods and molecular tools has been developed for genetic modification of at least ten different staphylococcal species to date. Here we review recent developments of various genetic tools and molecular methods for staphylococcal research, which include reporter systems and vectors for controllable gene expression, gene inactivation, gene essentiality testing, chromosomal integration and transposon delivery. It is furthermore illustrated how mutant strain construction by homologous or site-specific recombination benefits from sophisticated counterselection methods. The underlying genetic components have been shown to operate in wild-type staphylococci or modified chassis strains. Finally, possible future developments in the field of applied Staphylococcus genetics are highlighted.

Indexed as

Genetics, MicrobialMolecular BiologyStaphylococcus

Identifiers

PMID23378573
PMCPMC3709823

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.