ArticleJournal of bacteriology2013
Mating pair formation homologue TraG is a variable membrane protein essential for contact-independent type IV secretion of chromosomal DNA by Neisseria gonorrhoeae.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 24 citations in OpenAlex.
- The role of extracellular DNA inJournal of bacteriology · 2026Review
- DNA Transport through the Dynamic Type IV Secretion System.Infection and immunity · 2023Review
- Gonococcal Genetic Island in the GlobalMicroorganisms · 2023Article
- The Gonococcal Genetic Island defines distinct sub-populations ofMicrobial genomics · 2023Article
- Integrated conjugative plasmid drives high frequency chromosomal gene transfer inFrontiers in microbiology · 2023Article
- Comparative Whole-Genome Analysis ofFrontiers in cellular and infection microbiology · 2022Article
- Transcriptional and Translational Responsiveness of the Neisseria gonorrhoeae Type IV Secretion System to Conditions of Host Infections.Infection and immunity · 2021Article
- Expression, Localization, and Protein Interactions of the Partitioning Proteins in the Gonococcal Type IV Secretion System.Frontiers in microbiology · 2021Article
- Protein interactions within and between two F-type type IV secretion systems.Molecular microbiology · 2020Article
- Inhibiting bacterial secretion systems in the fight against antibiotic resistance.MedChemComm · 2019Review
- The Pathogenic Neisseria Use a Streamlined Set of Peptidoglycan Degradation Proteins for Peptidoglycan Remodeling, Recycling, and Toxic Fragment Release.Frontiers in microbiology · 2019Review
- Type IV secretion in Gram-negative and Gram-positive bacteria.Molecular microbiology · 2018Review
- The invasive Neisseria meningitidis MenC CC103 from Brazil is characterized by an accessory gene repertoire.Scientific reports · 2017Article
- The Rickettsia type IV secretion system: unrealized complexity mired by gene family expansion.Pathogens and disease · 2016Article
- Targeted mutagenesis of intergenic regions in the Neisseria gonorrhoeae gonococcal genetic island reveals multiple regulatory mechanisms controlling type IV secretion.Molecular microbiology · 2015Article
- Gene Transfer Efficiency in Gonococcal Biofilms: Role of Biofilm Age, Architecture, and Pilin Antigenic Variation.Journal of bacteriology · 2015Article
- Mobile DNA in the pathogenicMicrobiology spectrum · 2015Article
- TraK and TraB are conserved outer membrane proteins of the Neisseria gonorrhoeae Type IV secretion system and are expressed at low levels in wild-type cells.Journal of bacteriology · 2014Article
- Article
- Secretion of Chromosomal DNA by the Neisseria gonorrhoeae Type IV Secretion System.Current topics in microbiology and immunologyArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Neisseria gonorrhoeae uses a type IV secretion system (T4SS) to secrete chromosomal DNA into the surrounding milieu. The DNA is effective in transforming gonococci in the population, and this mechanism of DNA donation may contribute to the high degree of genetic diversity in this species. Similar to other F-like T4SSs, the gonococcal T4SS requires a putative membrane protein, TraG, for DNA transfer. In F-plasmid and related systems, the homologous protein acts in pilus production, mating pair stabilization, and entry exclusion. We characterized the localization, membrane topology, and variation of TraG in N. gonorrhoeae. TraG was found to be an inner-membrane protein with one large periplasmic region and one large cytoplasmic region. Each gonococcal strain carried one of three different alleles of traG. Strains that carried the smallest allele of traG were found to lack the peptidoglycanase gene atlA but carried a peptidoglycan endopeptidase gene in place of atlA. The purified endopeptidase degraded gonococcal peptidoglycan in vitro, cutting the peptide cross-links. Although the other two traG alleles functioned for DNA secretion in strain MS11, the smallest traG did not support DNA secretion. Despite the requirement for a mating pair stabilization homologue, static coculture transformation experiments demonstrated that DNA transfer was nuclease sensitive and required active uptake by the recipient, thus demonstrating that transfer occurred by transformation and not conjugation. Together, these results demonstrate the TraG acts in a process of DNA export not specific to conjugation and that different forms of TraG affect what substrates can be transported.
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