ArticleApplied and environmental microbiology2013
Rewiring Lactococcus lactis for ethanol production.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 60 citations in OpenAlex.
- Scrutinizing aApplied and environmental microbiology · 2024Article
- Rewiring the respiratory pathway of Lactococcus lactis to enhance extracellular electron transfer.Microbial biotechnology · 2023Article
- Plasmid-Based Gene Expression Systems for Lactic Acid Bacteria: A Review.Microorganisms · 2022Review
- Deciphering the Regulation of the Mannitol Operon Paves the Way for Efficient Production of Mannitol in Lactococcus lactis.Applied and environmental microbiology · 2021Article
- Efficient Production of Pyruvate Using Metabolically EngineeredFrontiers in bioengineering and biotechnology · 2020Article
- Synergy at work: linking the metabolism of two lactic acid bacteria to achieve superior production of 2-butanol.Biotechnology for biofuels · 2020Article
- Efficient production of α-acetolactate by whole cell catalytic transformation of fermentation-derived pyruvate.Microbial cell factories · 2019Article
- Culture fermentation ofJournal of food science and technology · 2019Article
- Genomics of Lactic Acid Bacteria for Glycerol Dissimilation.Molecular biotechnology · 2019Review
- Engineering of Biosynthesis Pathway and NADPH Supply for Improved L-5-Methyltetrahydrofolate Production byJournal of microbiology and biotechnology · 2019Article
- Systems Biology - A Guide for Understanding and Developing Improved Strains of Lactic Acid Bacteria.Frontiers in microbiology · 2019Review
- Genes and Gut Bacteria Involved in Luminal Butyrate Reduction Caused by Diet and Loperamide.Genes · 2017Article
- Article
- Synthesis of (3R)-acetoin and 2,3-butanediol isomers by metabolically engineered Lactococcus lactis.Scientific reports · 2016Article
- Lactobacillus casei as a biocatalyst for biofuel production.Journal of industrial microbiology & biotechnology · 2016Article
- A novel cell factory for efficient production of ethanol from dairy waste.Biotechnology for biofuels · 2016Article
- Adaptation of Lactococcus lactis to high growth temperature leads to a dramatic increase in acidification rate.Scientific reports · 2015Article
- Exploring the Genome of Cheese Starter Lactic Acid Bacterium Lactococcus lactis subsp. lactis CECT 4433.Genome announcements · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactic acid bacteria (LAB) are known for their high tolerance toward organic acids and alcohols (R. S. Gold, M. M. Meagher, R. Hutkins, and T. Conway, J. Ind. Microbiol. 10:45-54, 1992) and could potentially serve as platform organisms for production of these compounds. In this study, we attempted to redirect the metabolism of LAB model organism Lactococcus lactis toward ethanol production. Codon-optimized Zymomonas mobilis pyruvate decarboxylase (PDC) was introduced and expressed from synthetic promoters in different strain backgrounds. In the wild-type L. lactis strain MG1363 growing on glucose, only small amounts of ethanol were obtained after introducing PDC, probably due to a low native alcohol dehydrogenase activity. When the same strains were grown on maltose, ethanol was the major product and lesser amounts of lactate, formate, and acetate were formed. Inactivating the lactate dehydrogenase genes ldhX, ldhB, and ldh and introducing codon-optimized Z. mobilis alcohol dehydrogenase (ADHB) in addition to PDC resulted in high-yield ethanol formation when strains were grown on glucose, with only minor amounts of by-products formed. Finally, a strain with ethanol as the sole observed fermentation product was obtained by further inactivating the phosphotransacetylase (PTA) and the native alcohol dehydrogenase (ADHE).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.