ArticleApplied and environmental microbiology2013
Hha controls Escherichia coli O157:H7 biofilm formation by differential regulation of global transcriptional regulators FlhDC and CsgD.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 46 citations in OpenAlex.
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- The Transcription Factor CsgD Contributes to EngineeredInternational journal of molecular sciences · 2023Article
- Characterization of functional amyloid curli in biofilm formation of an environmental isolate Enterobacter cloacae SBP-8.Antonie van Leeuwenhoek · 2023Article
- Derivatives of Esculentin-1 Peptides as Promising Candidates for Fighting Infections fromAntibiotics (Basel, Switzerland) · 2022Article
- Genomewide transcriptional response of Escherichia coli O157:H7 to norepinephrine.BMC genomics · 2022Article
- Invasiveness ofPathogens (Basel, Switzerland) · 2021Article
- Massively parallel transposon mutagenesis identifies temporally essential genes for biofilm formation inMicrobial genomics · 2021Article
- Mucosal IFNγ production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle.Scientific reports · 2021Article
- Cellular and Mucosal Immune Responses Following Vaccination with Inactivated Mutant of Escherichia coli O157:H7.Scientific reports · 2019Article
- Comparative genomics reveals structural and functional features specific to the genome of a foodborne Escherichia coli O157:H7.BMC genomics · 2019Article
- RstA, a two-component response regulator, plays important roles in multiple virulence-associated processes in enterohemorrhagicGut pathogens · 2019Article
- Factors Involved in the Persistence of a Shiga Toxin-ProducingFrontiers in microbiology · 2018Article
- Involvement of Two-Component Signaling on Bacterial Motility and Biofilm Development.Journal of bacteriology · 2017Review
- Characterization of a Large Antibiotic Resistance Plasmid Found in Enteropathogenic Escherichia coli Strain B171 and Its Relatedness to Plasmids of Diverse E. coli and Shigella Strains.Antimicrobial agents and chemotherapy · 2017Article
- A starvation-induced regulator, RovM, acts as a switch for planktonic/biofilm state transition in Yersinia pseudotuberculosis.Scientific reports · 2017Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although molecular mechanisms promoting adherence of enterohemorrhagic Escherichia coli (EHEC) O157:H7 on epithelial cells are well characterized, regulatory mechanisms controlling biofilm formation are not fully understood. In this study, we demonstrate that biofilm formation in EHEC O157:H7 strain 86-24 is highly repressed compared to that in an isogenic hha mutant. The hha mutant produced large quantities of biofilm compared to the wild-type strain at 30°C and 37°C. Complementation of the hha mutant reduced the level of biofilm formation to that of the wild-type strain, indicating that Hha is a negative regulator of biofilm production. While swimming motility and expression of the flagellar gene fliC were significantly reduced, the expression of csgA (encoding curlin of curli fimbriae) and the ability to bind Congo red were significantly enhanced. The expression of both fliC and csgA and the phenotypes of motility and curli production affected by these two genes, respectively, were restored to wild-type levels in the complemented hha mutant. The csgA deletion abolished biofilm formation in the hha mutant and wild-type strain, and csgA complementation restored biofilm formation to these strains, indicating the importance of csgA and curli in biofilm formation. The regulatory effects of Hha on flagellar and curli gene expression appear to occur via the induction and repression of FlhDC and CsgD, as demonstrated by reduced flhD and increased csgD transcription in the hha mutant, respectively. In gel shift assays Hha interacted with flhDC and csgD promoters. In conclusion, Hha regulates biofilm formation in EHEC O157:H7 by differential regulation of FlhDC and CsgD, the global regulators of motility and curli production, respectively.
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