ArticlePflugers Archiv : European journal of physiology2013
Thermal inactivation of volume-sensitive K⁺,Cl⁻ cotransport and plasma membrane relief changes in human erythrocytes.
Article in Pflugers Archiv : European journal of physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed.
- Heat damage of cytoskeleton in erythrocytes increases membrane roughness and cell rigidity.Journal of biological physics · 2019Article
- KJournal of hematology & oncology · 2019Review
- Hypotonicity-induced cell swelling activates TRPA1.The journal of physiological sciences : JPS · 2018Article
- The effect of a high frequency electromagnetic field in the microwave range on red blood cells.Scientific reports · 2017Article
- Salt and osmosensing: role of cytoplasmic hydrogel.Pflugers Archiv : European journal of physiology · 2015Review
- Temperature-induced inactivation of cytoplasmic biogel osmosensing properties is associated with suppression of regulatory volume decrease in A549 cells.The Journal of membrane biology · 2014Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, we reported that in mammalian erythrocytes irreversible annealing of spectrin heterodimers at 49-50 °C abolished cell volume-dependent regulation of ion carriers, thus suggesting an implication of a two-dimensional (2D) membrane carcass in volume sensing and/or signal transduction. To further examine this hypothesis, we employed atomic force microscopy. This method revealed folded membrane relief of fixed human erythrocytes with an average wave height of 3-5 nm covered by globular structures with a diameter of 40-50 nm and an average height of 1-2 nm. Erythrocyte swelling caused by reduction of medium osmolality decreased the height of membrane surface waves by 40 % and increased K(+),Cl(-) cotransport by approximately sixfold. Both volume-sensitive changes of membrane relief and activity of K(+),Cl(-) cotransporter were abolished by a 10-min preincubation at 50 °C. Our results strongly suggest that volume-dependent alterations of the human erythrocyte membrane relief are caused by reorganization of the 2D spectrin-actin network contributing to regulation of the activity of volume-sensitive ion transporters.
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Registered trials
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