Evidence map›Paper›PMID 23376832›Full record

ArticleAmerican journal of physiology. Heart and circulatory physiology2013

Cardiac myocyte exosomes: stability, HSP60, and proteomics.

Z A Malik, K S Kott, A J Poe, T Kuo, L Chen, K W Ferrara, A A Knowlton

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Heart and circulatory physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 123 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
123citing papers in PubMed, 1 pooled it
6.6field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

123 citing papers in PubMed, 1 synthesis or guideline pooled it, 206 citations in OpenAlex.

  1. Pooled it
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  13. Role of Exosomes in Cardiovascular Diseases.Reviews in cardiovascular medicine · 2024
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63 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Z A MalikMolecular and Cellular Cardiology, Department of Medicine, University of California, Davis, CA 95616, USA.
K S Kott
A J Poe
T Kuo
L Chen
K W Ferrara
A A Knowlton
University of California, Davis · USUniversity of California Davis Medical Center · US

Funding

Human Cardiomyopathy and HSP60R01HL077281 · NHLBI · UNIVERSITY OF CALIFORNIA DAVIS · PI KNOWLTON, ANNE A · 2004 to 2012
$3.1M
HSPs, Inflammatory Response and Cardiovascular DiseaseR01HL079071 · NHLBI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI KNOWLTON, ANNE A · 2006 to 2014
$2.7M
NHLBI NIH HHS HL-077281NHLBI NIH HHS HL079071
6 · The paper itself

Abstract

Exosomes, which are 50- to 100-nm-diameter lipid vesicles, have been implicated in intercellular communication, including transmitting malignancy, and as a way for viral particles to evade detection while spreading to new cells. Previously, we demonstrated that adult cardiac myocytes release heat shock protein (HSP)60 in exosomes. Extracellular HSP60, when not in exosomes, causes cardiac myocyte apoptosis via the activation of Toll-like receptor 4. Thus, release of HSP60 from exosomes would be damaging to the surrounding cardiac myocytes. We hypothesized that 1) pathological changes in the environment, such as fever, change in pH, or ethanol consumption, would increase exosome permeability; 2) different exosome inducers would result in different exosomal protein content; 3) ethanol at "physiological" concentrations would cause exosome release; and 4) ROS production is an underlying mechanism of increased exosome production. We found the following: first, exosomes retained their protein cargo under different physiological/pathological conditions, based on Western blot analyses. Second, mass spectrometry demonstrated that the protein content of cardiac exosomes differed significantly from other types of exosomes in the literature and contained cytosolic, sarcomeric, and mitochondrial proteins. Third, ethanol did not affect exosome stability but greatly increased the production of exosomes by cardiac myocytes. Fourth, ethanol- and hypoxia/reoxygenation-derived exosomes had different protein content. Finally, ROS inhibition reduced exosome production but did not completely inhibit it. In conclusion, exosomal protein content is influenced by the cell source and stimulus for exosome formation. ROS stimulate exosome production. The functions of exosomes remain to be fully elucidated.

Indexed as

AnimalsChaperonin 60EthanolExosomesHypoxiaMaleMitochondrial ProteinsMyocytes, CardiacProtein StabilityProteomeProteomicsRatsRats, Sprague-DawleyReactive Oxygen SpeciesChaperonin 60EthanolMitochondrial ProteinsProteomeReactive Oxygen Species

Identifiers

PMID23376832
PMCPMC3625894
OpenAlexW2016834327

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.