ArticleThe international journal of biochemistry & cell biology2013
Isolation of a calcium-binding protein of the acrosomal membrane of bovine spermatozoa.
Article in The international journal of biochemistry & cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 13 citations in OpenAlex.
- Identification and Characterization of TEX101 in Bovine Epididymal Spermatozoa.Biochemistry research international · 2014Article
- Comparative testicular transcriptome of wild type and globozoospermic Dpy19l2 knock out mice.Basic and clinical andrology · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The mammalian sperm acrosome reaction is a calcium-dependent exocytotic event characterized by extensive fusion between the plasma and the outer acrosomal membrane. The mechanisms by which elevation of cytosolic calcium initiates the membrane fusion process are not understood and the present study was undertaken to identify calcium-binding proteins in the acrosomal membrane (AM) of bovine spermatozoa. Sperm heads, purified from sonicated spermatozoa, were used to isolate an acrosomal membrane-enriched fraction on Percoll density gradients. Using SDS-PAGE and a (45)Ca(2+)-blot overlay assay, calcium-binding proteins of 64, 45, 43, and 39kDa were identified in the AM enriched fraction. Phase separation analysis with Triton X-114 identified the 64kDa polypeptide as an integral membrane protein. The 64kDa polypeptide was purified and utilized to prepare a polyclonal antiserum. Both light and electron microscopic immunocytochemistry demonstrated that the protein was distributed throughout all domains of the acrosomal membrane. These results identify a 64kDa calcium-binding integral membrane protein of the mammalian acrosome. Its potential function in calcium-dependent membrane fusion events of the acrosome reaction and in fertilization is discussed.
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Registered trials
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