ArticleActa biomaterialia2013
Three-dimensional hMSC motility within peptide-functionalized PEG-based hydrogels of varying adhesivity and crosslinking density.
Article in Acta biomaterialia, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
- A Behavior-Integrated Potency Assay for Quantitative Evaluation of Extracellular Matrix Remodeling by Mesenchymal Stem/Stromal Cells.Biotechnology and bioengineering · 2026Article
- The Intersecting Physical Mechanisms That Regulate Cell Viability in 3D Synthetic Hydrogels.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- A technique to create hydrogels with tethered concentration gradients of moleculesSoft matter · 2026Article
- Review
- Patterning of anisotropic physical cues in granular PEG hydrogel composites using magnetic templating.Industrial & engineering chemistry research · 2025Article
- Advances in Nanohybrid Hydrogels for Wound Healing: From Functional Mechanisms to Translational Prospects.Gels (Basel, Switzerland) · 2025Review
- Design and Characterization of Thioester Networks with Adaptable and Enzymatically Degradable Cross-Links.Macromolecules · 2025Article
- Tunable Bicontinuous Macroporous Cell Culture Scaffolds via Kinetically Controlled Phase Separation.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- A Rheological Study on the Effect of Tethering Pro- and Anti-Inflammatory Cytokines into Hydrogels on Human Mesenchymal Stem Cell Migration, Degradation, and Morphology.Biomacromolecules · 2024Article
- 3D Bioprinting Highly Elastic PEG-PCL-DA Hydrogel for Soft Tissue Fabrication and Biomechanical Stimulation.Advanced functional materials · 2024Article
- Design approaches for 3D cell culture and 3D bioprinting platforms.Biophysics reviews · 2024Review
- In Situ Synthesis and Self-Assembly of Peptide-PEG Conjugates: A Facile Method for the Construction of Fibrous Hydrogels.Biomacromolecules · 2024Article
- Chemical Modification of Human Decellularized Extracellular Matrix for Incorporation into Phototunable Hybrid-Hydrogel Models of Tissue Fibrosis.ACS applied materials & interfaces · 2023Article
- OP3-4 peptide sustained-release hydrogel inhibits osteoclast formation and promotes vascularization to promote bone regeneration in a rat femoral defect model.Bioengineering & translational medicine · 2023Article
- Convergence of Biofabrication Technologies and Cell Therapies for Wound Healing.Pharmaceutics · 2022Review
- Alveolar epithelial cells and microenvironmental stiffness synergistically drive fibroblast activation in three-dimensional hydrogel lung models.Biomaterials science · 2022Article
- Current strategies for enhancement of the bioactivity of artificial ligaments: A mini-review.Journal of orthopaedic translation · 2022Review
- Measuring the Effects of Cytokines on the Modification of Pericellular Rheology by Human Mesenchymal Stem Cells.ACS biomaterials science & engineering · 2021Article
- Bone Regeneration Using MMP-Cleavable Peptides-Based Hydrogels.Gels (Basel, Switzerland) · 2021Review
- Alginate-Based Bioinks for 3D Bioprinting and Fabrication of Anatomically Accurate Bone Grafts.Tissue engineering. Part A · 2021Article
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Authors and funding
2 authors.
Funding
Abstract
Human mesenchymal stem cell (hMSC) migration and recruitment play a critical role during bone fracture healing. Within the complex three-dimensional (3-D) in vivo microenvironment, hMSC migration is regulated through a myriad of extracellular cues. Here, we use a thiol-ene photopolymerized hydrogel to recapitulate structural and bioactive inputs in a tunable manner to understand their role in regulating 3-D hMSC migration. Specifically, peptide-functionalized poly(ethylene glycol) hydrogels were used to encapsulate hMSC while varying the crosslinking density, from 0.18±0.02 to 1.60±0.04 mM, and the adhesive ligand density, from 0.001 to 1.0 mM. Using live-cell videomicroscopy, migratory cell paths were tracked and fitted to a Persistent Random Walk model. It was shown that hMSC migrating through the lowest crosslinking density and highest adhesivity had more sustained polarization, higher migrating speeds (17.6±0.9 μm h(-1)) and higher cell spreading (elliptical form factor=3.9±0.2). However, manipulation of these material properties did not significantly affect migration persistence. Further, there was a monotonic increase in cell speed and spreading with increasing adhesivity that showed a lack of the biphasic trend seen in 2-D cell migration. Immunohistochemistry showed well-formed actin fibers and β1 integrin staining at the ends of stress fibers. This thiol-ene platform provides a highly tunable substrate to characterize 3-D hMSC migration that can be applied as an implantable cell carrier platform or for the recruitment of endogenous hMSC in vivo.
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