ArticleAnalytical biochemistry2013
Synthesis and characterization of a SIRT6 open tubular column: predicting deacetylation activity using frontal chromatography.
Article in Analytical biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- Studies and analysis of drug-target interactions by affinity chromatography and related techniques: A review.Journal of pharmaceutical analysis · 2026Review
- Microscale Affinity Chromatography for Biointeraction Analysis: Strategies, Principles and Applications.Journal of separation science · 2026Review
- SIRT6 in Regulation of Mitochondrial Damage and Associated Cardiac Dysfunctions: A Possible Therapeutic Target for CVDs.Cardiovascular toxicology · 2024Review
- A Comprehensive Analysis into the Therapeutic Application of Natural Products as SIRT6 Modulators in Alzheimer's Disease, Aging, Cancer, Inflammation, and Diabetes.International journal of molecular sciences · 2021Review
- Emerging roles of SIRT6 in human diseases and its modulators.Medicinal research reviews · 2021Review
- Recent Advances in Supramolecular Affinity Separations: Affinity Chromatography and Related Methods.Advances in chromatography · 2021Article
- Multitargeted Effects of Vitexin and Isovitexin on Diabetes Mellitus and Its Complications.TheScientificWorldJournal · 2021Review
- Structural basis for the activation and inhibition of Sirtuin 6 by quercetin and its derivatives.Scientific reports · 2019Article
- Sirtuins and SIRT6 in Carcinogenesis and in Diet.International journal of molecular sciences · 2019Review
- Natural polyphenols as sirtuin 6 modulators.Scientific reports · 2018Article
- Analysis of stereoselective drug interactions with serum proteins by high-performance affinity chromatography: A historical perspective.Journal of pharmaceutical and biomedical analysis · 2017Review
- Analysis of Biological Interactions by Affinity Chromatography: Clinical and Pharmaceutical Applications.Clinical chemistry · 2017Review
- Comparison of analytical techniques for the identification of bioactive compounds from natural products.Natural product reports · 2016Review
- Dietary phytochemicals as epigenetic modifiers in cancer: Promise and challenges.Seminars in cancer biology · 2016Review
- Development and characterization of the α3β4α5 nicotinic receptor cellular membrane affinity chromatography column and its application for on line screening of plant extracts.Journal of chromatography. A · 2016Article
- Targeting Anti-Cancer Active Compounds: Affinity-Based Chromatographic Assays.Current pharmaceutical design · 2016Review
- N-Acylethanolamines Bind to SIRT6.Chembiochem : a European journal of chemical biology · 2016Article
- Sirtuin 6 (SIRT6) Activity Assays.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Anticancer potential of the histone deacetylase inhibitor-like effects of flavones, a subclass of polyphenolic compounds: a review.Molecular biology reports · 2015Review
- Development of enhanced capacity affinity microcolumns by using a hybrid of protein cross-linking/modification and immobilization.Journal of chromatography. A · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
SIRT6 is a histone deacetylase that has been proposed as a potential therapeutic target for metabolic disorders and the prevention of age-associated diseases. Thus the identification of compounds that modulate SIRT6 activity could be of great therapeutic importance. We have previously reported on the identification of quercetin and vitexin as SIRT6 inhibitors, using SIRT6-coated magnetic beads. In this study, we have immobilized SIRT6 onto the surface of an open tubular capillary and characterized the quercetin binding site using frontal displacement chromatography. Structurally related flavonoids were tested for their activity on SIRT6, including apigenin, naringenin, luteolin, and kaempferol. In addition to obtaining their binding activity using frontal affinity chromatographic techniques, we also ranked the compounds based on their ability to displace quercetin. The data suggest that a single displacement curve is representative of the enzymatic activity of the tested ligand. In addition, using the inhibition data obtained in this study, we developed a preliminary pharmacophore model that confirmed the experimental data.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.