ArticleThe Journal of allergy and clinical immunology2013
Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis.
Article in The Journal of allergy and clinical immunology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
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Who cites it
93 citing papers in PubMed, 188 citations in OpenAlex.
- Context-dependent regulation of mast cell-mediated food allergy in C57BL/6J mice.The Journal of allergy and clinical immunology · 2026Article
- Hidden immune memory niches in inflammatory skin diseases.bioRxiv : the preprint server for biology · 2026Article
- Investigating the consequences of tape-stripping in ovalbumin-induced allergic mouse model: From physiology to gene regulation.Biochemistry and biophysics reports · 2026Article
- Hyaluronic Acid ProfhiloMedicina (Kaunas, Lithuania) · 2026Article
- Dietary Antigen Interaction with Intestinal Epithelial Cells.Current allergy and asthma reports · 2026Review
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- Constitutively high levels of endogenous soluble ST2 inhibit food allergic responses in mice.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Targeted therapies in atopic dermatitis: toward endotype-driven precision medicine and disease modification.Frontiers in immunology · 2026Review
- Insights Into Complex Murine Models of Allergy and Anaphylaxis: The Central Role of IgE and Mast Cells in Advancing Human Therapies.European journal of immunology · 2025Review
- S. aureus exposure during cutaneous antigen sensitization causes basophil- and interleukin-4-dependent exaggerated food anaphylaxis.Immunity · 2025Article
- Food allergy: begin at the skin, end at the mast cell?Nature reviews. Immunology · 2025Review
- Oral and Skin Sensitisation to Peanut Show Different Immunological Features in Brown Norway Rats.Scandinavian journal of immunology · 2025Article
- Decoding Early Clues: Immune Mechanisms, Prevention, Diagnosis, and Treatment of IgE-Mediated Peanut and Tree Nut Allergy in Children.Biomedicines · 2025Review
- Intestinal mast cell-derived leukotrienes mediate the anaphylactic response to ingested antigens.Science (New York, N.Y.) · 2025Article
- DOCK8 in T cells promotes Th17 and Treg cell functionality to restrain mucosal mast cells and limit susceptibility to oral anaphylaxis.Immunity · 2025Article
- Early-Life Allergen Exposure and Its Influence on Risk of Atopic Disease.The journal of allergy and clinical immunology. In practice · 2025Review
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- Skin damage signals mediate allergic sensitization to spatially unlinked antigen.Science immunology · 2025Article
- Review
33 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundSensitization to food antigen can occur through cutaneous exposure.
objectiveWe sought to test the hypothesis that epicutaneous sensitization with food antigen predisposes to IgE-mediated anaphylaxis on oral allergen challenge.
methodsBALB/c mice were epicutaneously sensitized by repeated application of ovalbumin (OVA) to tape-stripped skin over 7 weeks or orally immunized with OVA and cholera toxin (CT) weekly for 8 weeks and then orally challenged with OVA. Body temperature was monitored, and serum mouse mast cell protease 1 levels were determined after challenge. Tissue mast cell (MC) counts were examined by using chloroacetate esterase staining. Levels of serum OVA-specific IgE and IgG(1) antibodies and cytokines in supernatants of OVA-stimulated splenocytes were measured by means of ELISA. Serum IL-4 levels were measured by using an in vivo cytokine capture assay.
resultsEpicutaneously sensitized mice exhibited expansion of connective tissue MCs in the jejunum, increased serum IL-4 levels, and systemic anaphylaxis after oral challenge, as evidenced by decreased body temperature and increased serum mouse mast cell protease 1 levels. Intestinal MC expansion and anaphylaxis were IgE dependent because they did not occur in epicutaneously sensitized IgE(-/-) mice. Mice orally immunized with OVA plus CT did not have increased serum IL-4 levels, expanded intestinal MCs, or anaphylaxis after oral challenge, despite OVA-specific IgE levels and splenocyte cytokine production in response to OVA stimulation, which were comparable with those of epicutaneously sensitized mice.
conclusionEpicutaneously sensitized mice, but not mice orally immunized with antigen plus CT, have expansion of intestinal MCs and IgE-mediated anaphylaxis after single oral antigen challenge. IgE is necessary but not sufficient for food anaphylaxis, and MC expansion in the gut can play an important role in the development of anaphylaxis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.