ArticleTissue engineering. Part C, Methods2013
Sustainable three-dimensional tissue model of human adipose tissue.
Article in Tissue engineering. Part C, Methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 72 citations in OpenAlex.
- Adipose Tissue Engineering Biomaterials: Smart Scaffolds, Vascularization, and Clinical Frontiers.Biomolecules · 2026Review
- 2D and 3D In Vitro Co-culture for Cancer and Bone Cell Interaction Studies.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Development and analysis of scaffold-free adipose spheroids.Adipocyte · 2024Article
- Mesenchymal Stem Cell-based Scaffolds in Regenerative Medicine of Dental Diseases.Stem cell reviews and reports · 2024Review
- Repurposing Decellularized Lung to Generate Vascularized Fat.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Optimization and validation of a fat-on-a-chip model for non-invasive therapeutic drug discovery.Frontiers in bioengineering and biotechnology · 2024Article
- Clinical Applicability of Visible Light-Mediated Cross-linking for Structural Soft Tissue Reconstruction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Development of a More Environmentally Friendly Silk Fibroin Scaffold for Soft Tissue Applications.Journal of functional biomaterials · 2023Article
- High-throughput drug screening models of mature adipose tissues which replicate the physiology of patients' Body Mass Index (BMI).Bioactive materials · 2022Article
- Hypoxia induces stress fiber formation in adipocytes in the early stage of obesity.Scientific reports · 2021Article
- Mechanical Memory Impairs Adipose-Derived Stem Cell (ASC) Adipogenic Capacity After Long-TermCellular and molecular bioengineering · 2021Article
- Engineering a 3D Vascularized Adipose Tissue Construct Using a Decellularized Lung Matrix.Biomimetics (Basel, Switzerland) · 2021Article
- 3D Adipose Tissue Culture Links the Organotypic Microenvironment to Improved Adipogenesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- The Materiobiology of Silk: Exploring the Biophysical Influence of Silk Biomaterials on Directing Cellular Behaviors.Frontiers in bioengineering and biotechnology · 2021Review
- Endothelial cell crosstalk improves browning but hinders white adipocyte maturation in 3D engineered adipose tissue.Integrative biology : quantitative biosciences from nano to macro · 2020Article
- Mechanisms of action, chemical characteristics, and model systems of obesogens.BMC biomedical engineering · 2020Review
- Human Adipose Derived Cells in Two- and Three-Dimensional Cultures: Functional Validation of an In Vitro Fat Construct.Stem cells international · 2020Article
- iTRAQ-Based Quantitative Proteomic Comparison of 2D and 3D Adipocyte Cell Models Co-cultured with Macrophages Using Online 2D-nanoLC-ESI-MS/MS.Scientific reports · 2019Article
- The importance of the neuro-immuno-cutaneous system on human skin equivalent design.Cell proliferation · 2019Review
- Biomaterials to Mimic and Heal Connective Tissues.Advanced materials (Deerfield Beach, Fla.) · 2019Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The need for physiologically relevant sustainable human adipose tissue models is crucial for understanding tissue development, disease progression, in vitro drug development and soft tissue regeneration. The coculture of adipocytes differentiated from human adipose-derived stem cells, with endothelial cells, on porous silk protein matrices for at least 6 months is reported, while maintaining adipose-like outcomes. Cultures were assessed for structure and morphology (Oil Red O content and CD31 expression), metabolic functions (leptin, glycerol production, gene expression for GLUT4, and PPARγ) and cell replication (DNA content). The cocultures maintained size and shape over this extended period in static cultures, while increasing in diameter by 12.5% in spinner flask culture. Spinner flask cultures yielded improved adipose tissue outcomes overall, based on structure and function, when compared to the static cultures. This work establishes a tissue model system that can be applied to the development of chronic metabolic dysfunction systems associated with human adipose tissue, such as obesity and diabetes, due to the long term sustainable functions demonstrated here.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.