ArticlePloS one2013
Distribution and genetic characterizations of Cryptosporidium spp. in pre-weaned dairy calves in Northeastern China's Heilongjiang Province.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 2 of them syntheses that pooled it.
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Who cites it
53 citing papers in PubMed, 2 syntheses or guidelines pooled it, 109 citations in OpenAlex.
- Prevalence ofFrontiers in cellular and infection microbiology · 2021Pooled it
- A retrospective epidemiological analysis of human Cryptosporidium infection in China during the past three decades (1987-2018).PLoS neglected tropical diseases · 2020Pooled it
- Seasonal Prevalence and Molecular Characterization ofLife (Basel, Switzerland) · 2026Article
- Prevalence of cryptosporidiosis and other enteric pathogens in calves in France: effects of rotavirus, coronavirus and E. coli vaccination and transmission routes.Veterinary research · 2026Article
- Prevalence and distribution of Cryptosporidium spp. in cattle in central and western Inner Mongolia, China.BMC microbiology · 2025Article
- Review
- Prevalence and molecular characterization of Cryptosporidium spp. in pre-weaned diarrheic dairy calves and their bedding materials in northern China.Parasitology research · 2024Article
- Prevalence and Molecular Characterization ofAnimals : an open access journal from MDPI · 2024Article
- Molecular characterization of common zoonotic protozoan parasites and bacteria causing diarrhea in dairy calves in Ningxia Hui Autonomous Region, China.Parasite (Paris, France) · 2024Article
- Prevalence and molecular characterization of Cryptosporidium spp. in dairy and beef cattle in Shanxi, China.Parasitology research · 2023Article
- Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle in Central Inner Mongolia, Northern China.BMC veterinary research · 2023Article
- Molecular evaluation of Cryptosporidium spp. among breeding calves of Lorestan province Western Iran.Veterinary medicine and science · 2023Article
- First report ofFrontiers in veterinary science · 2023Article
- Article
- Genotyping of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi from sheep and goats in China.BMC veterinary research · 2022Article
- Occurrence and Molecular Characterization ofAnimals : an open access journal from MDPI · 2022Article
- An Update on ZoonoticAnimals : an open access journal from MDPI · 2021Review
- Molecular prevalence and characterization of Cryptosporidium in domestic free-range poultry in Anhui Province, China.Parasitology research · 2021Article
- Prevalence and multilocus genotyping of Cryptosporidium spp. in cattle in Jiangxi Province, southeastern China.Parasitology research · 2021Article
- The Age-RelatedAnimals : an open access journal from MDPI · 2021Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCryptosporidium spp. are common parasites of humans and animals. Farm animals, especially pre-weaned calves, are considered to be one of main animal reservoir hosts of Cryptosporidium in the transmission of human cryptosporidiosis. The aim of this study was to determine the distribution and genotypes of Cryptosporidium spp. in pre-weaned calves using molecular tools and to assess zoonotic transmission and elucidate the public health significance in northeastern China. METHODOLOGY/PRINCIPAL
findingsA total of 151 fecal specimens from pre-weaned calves were collected in Heilongjiang Province and were screened for Cryptosporidium by PCR. The average prevalence of Cryptosporidium was 47.68% (72/151). Cryptosporidium spp. were characterized by DNA sequencing of the small subunit (SSU) rRNA gene and the 60-kDa glycoprotein (gp60) gene. Based on the SSU rRNA gene, five Cryptosporidium spp. were identified, including C. bovis (n = 34), C. andersoni (n = 26), C. ryanae (n = 5), C. meleagridis (n = 5) and C. parvum (n = 2). The SSU rRNA nucleotide sequences were identical to each other, respectively, within C. ryanae, C. parvum, C. meleagridis and C. andersoni. Four types of C. bovis were found in the SSU rRNA gene, with two novel types. The gp60 gene was successfully sequenced in one C. parvum isolate and three C. meleagridis isolates, with IIdA19G1 for C. parvum and IIIeA22G2R1 for C. meleagridis. CONCLUSION/SIGNIFICANCE: Molecular analysis indicates that Cryptosporidium spp. are endemic in pre-weaned calves in Heilongjiang Province. The findings of C. parvum and C. meleagridis suggested the possibility of zoonotic transmission and public health significance. The transmission dynamics of C. parvum and C. meleagridis needed to be clarified by further molecular epidemiologic studies from humans and animals. Whether calves could act as the natural reservoirs of C. meleagridis needed to be confirmed by more systematic experimental infection studies.
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