ArticleWorld journal of gastroenterology2013
Helicobacter pylori tumor necrosis factor-α inducing protein promotes cytokine expression via nuclear factor-κB.
Article in World journal of gastroenterology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Sphingomonas paucimobilis-Driven Epithelial-Endothelial Transition in Adenomyosis Pathogenesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Correlation between dyslipidaemia and gastric cancer: pathogenesis to prevention and treatment strategies.Lipids in health and disease · 2025Review
- More powerful dysregulation of Helicobacter pylori East Asian-type CagA on intracellular signalings.BMC microbiology · 2024Article
- Article
- Review of dietary patterns and gastric cancer risk: epidemiology and biological evidence.Frontiers in oncology · 2024Review
- Helicobacter pylori-activated fibroblasts as a silent partner in gastric cancer development.Cancer metastasis reviews · 2023Review
- Reactive Oxygen Species andAntioxidants (Basel, Switzerland) · 2023Review
- Inflammation and Gastric Cancer.Diseases (Basel, Switzerland) · 2022Review
- Association of Progranulin Gene Expression from Dyspeptic Patients with VirulentMicroorganisms · 2022Article
- Role of TNF-α-Inducing Protein Secreted byToxins · 2021Review
- MALDI-TOF MS and 16S RNA Identification of Culturable Gastric Microbiota: Variability Associated with the Presence ofMicroorganisms · 2020Article
- Review
- Clinical and laboratory importance of detectingPrzeglad gastroenterologiczny · 2020Review
- The Possible Role ofFrontiers in oncology · 2019Review
- NF-κB Signaling in Gastric Cancer.Toxins · 2017Review
- Linkages between oral commensal bacteria and atherosclerotic plaques in coronary artery disease patients.NPJ biofilms and microbiomes · 2016Article
- Metabolic consequences of Helicobacter pylori infection and eradication.World journal of gastroenterology · 2014Review
- Helicobacter pylori infection inhibits phagocyte clearance of apoptotic gastric epithelial cells.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo study the effects of Helicobacter pylori (H. pylori) tumor necrosis factor-α (TNF) inducing protein (Tip-α) on cytokine expression and its mechanism.
methodsWe cloned Tip-α from the H. pylori strain 26695, transformed Escherichia coli with an expression plasmid, and then confirmed the expression product by Western blotting. Using different concentrations of Tip-α that affected SGC7901 and GES-1 cells at different times, we assessed cytokine levels using enzyme-linked immunosorbent assay. We blocked SGC7901 cells with pyrrolidine dithiocarbamate (PDTC), a specific inhibitor of nuclear factor κB (NF-κB). We then detected interleukin (IL)-1β and TNF-α levels in SGC7901 cells.
resultsWestern blot analysis using an anti-Tip-α antibody revealed a 23-kDa protein, which indicated that recombinant Tip-α protein was recombined successfully. The levels of IL-1β, IL-8 and TNF-α were significantly higher following Tip-α interference, whether GES-1 cells or SGC-7901 cells were used (P < 0.05). However, the levels of cytokines (including IL-1β, IL-8 and TNF-α) secreted by SGC-7901 cells were greater than those secreted by GES-1 cells following treatment with Tip-α at the same concentration and for the same duration (P < 0.05). After blocking NF-κB with PDTC, the cells (GES-1 cells and SGC-7901 cells) underwent interference with Tip-α. We found that IL-1β and TNF-α levels were significantly decreased compared to cells that only underwent Tip-α interference (P < 0.05).
conclusionTip-α plays an important role in cytokine expression through NF-κB.
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