ArticleParasitology research2013
Expression of Toxoplasma gondii dense granule protein7 (GRA7) in Eimeria tenella.
Article in Parasitology research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Genetic manipulation for the non-model protozoaniScience · 2025Review
- Global profiling of protein S-palmitoylation in the second-generation merozoites of Eimeria tenella.Parasitology research · 2024Article
- Article
- Eimeria proteins: order amidst disorder.Parasites & vectors · 2022Review
- Dynamically expressed genes provide candidate viability biomarkers in a model coccidian.PloS one · 2021Article
- Towards Innovative Design and Application of RecombinantInfection and immunity · 2020Review
- Characterization of novel microneme adhesive repeats (MAR) in Eimeria tenella.Parasites & vectors · 2017Article
- Eimeria tenella protein trafficking: differential regulation of secretion versus surface tethering during the life cycle.Scientific reports · 2017Article
- Transfection of Eimeria mitis with yellow fluorescent protein as reporter and the endogenous development of the transgenic parasite.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dense granules are specialized secretory organelles of Apicomplexa parasites; the dense granule (GRA) proteins are believed to play a role in intracellular survival and the nutrient/waste exchange mechanism with the host cell. Until now, limited information is available concerning the characterization of GRA proteins in Eimeria. Eimeria tenella and Toxoplasma gondii are apicomplexan protozoa and share many similarities in biology and genomics. We hypothesized that GRA proteins from T. gondii could be expressed and have a similar function in E. tenella. To confirm the expression and localization of the GRA protein in T. gondii and E. tenella, a transient transfection strategy was used to express T. gondii GRA7 tagged with yellow fluorescent protein (YFP) (GRA7-YFP); T. gondii tachyzoites were transfected with the plasmid pTgtubGRA7-YFP/sagCAT, and E. tenella sporozoites were transfected with the pEtmic1GRA7-YFP/act construct. The results show that fluorescence can be expressed mainly into the parasitophorous vacuoles (PVs) of the T. gondii. GRA7 of T. gondii can also be expressed in E. tenella and can lead the fluorescence protein into the PVs of the parasites and the cavity of the sporocysts. As for the extracellular stage, YFP gathered to form small particles in the released merozoites and sporozoites, suggesting a localization of the secretory organelles of E. tenella. These results suggest that GRA proteins have a conserved function across species of Apicomplexa in targeting proteins to the PVs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.