ArticleMolecular neurobiology2013
Induced formation and maturation of acetylcholine receptor clusters in a defined 3D bio-artificial muscle.
Article in Molecular neurobiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- A knock down strategy for rapid, generic, and versatile modelling of muscular dystrophies in 3D-tissue-engineered-skeletal muscle.Skeletal muscle · 2024Article
- Changes in Elastic Moduli of Fibrin Hydrogels Within the Myogenic Range Alter Behavior of Murine C2C12 and Human C25 Myoblasts Differently.Frontiers in bioengineering and biotechnology · 2022Article
- Biomimetic glycosaminoglycan-based scaffolds improve skeletal muscle regeneration in a Murine volumetric muscle loss model.Bioactive materials · 2021Article
- Remodeling of the skeletal muscle and postsynaptic component after short-term joint immobilization and aquatic training.Histochemistry and cell biology · 2020Article
- Skeletal Muscle Tissue Engineering: Biomaterials-Based Strategies for the Treatment of Volumetric Muscle Loss.Bioengineering (Basel, Switzerland) · 2020Review
- Neuromuscular disease modeling on a chip.Disease models & mechanisms · 2020Review
- Polymer- and Hybrid-Based Biomaterials for Interstitial, Connective, Vascular, Nerve, Visceral and Musculoskeletal Tissue Engineering.Polymers · 2020Review
- Engineering Biomimetic Materials for Skeletal Muscle Repair and Regeneration.Advanced healthcare materials · 2019Review
- Human tissue-engineered skeletal muscle: a novel 3D in vitro model for drug disposition and toxicity after intramuscular injection.Scientific reports · 2018Article
- Vascularized microfluidic organ-chips for drug screening, disease models and tissue engineering.Current opinion in biotechnology · 2018Review
- Bioinspired Three-Dimensional Human Neuromuscular Junction Development in Suspended Hydrogel Arrays.Tissue engineering. Part C, Methods · 2018Article
- Biomaterials for skeletal muscle tissue engineering.Current opinion in biotechnology · 2017Review
- Striated muscle function, regeneration, and repair.Cellular and molecular life sciences : CMLS · 2016Review
- Design, evaluation, and application of engineered skeletal muscle.Methods (San Diego, Calif.) · 2016Review
- Achieving Acetylcholine Receptor Clustering in Tissue-Engineered Skeletal Muscle ConstructsFrontiers in pharmacology · 2016Article
- Endothelial Network Formation Within Human Tissue-Engineered Skeletal Muscle.Tissue engineering. Part A · 2015Article
- Biomaterial-based delivery for skeletal muscle repair.Advanced drug delivery reviews · 2015Review
- Skeletal muscle tissue engineering: strategies for volumetric constructs.Frontiers in physiology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
Abstract
Dysfunction of the neuromuscular junction is involved in a wide range of muscular diseases. The development of neuromuscular junction through which skeletal muscle is innervated requires the functional modulation of acetylcholine receptor (AchR) clustering on myofibers. However, studies on AchR clustering in vitro are mostly done on monolayer muscle cell culture, which lacks a three-dimensional (3D) structure, a prominent limitation of the two-dimensional (2D) system. To enable a better understanding on the structure-function correlation underlying skeletal muscle innervation, a muscle system with a well-defined geometry mimicking the in vivo muscular setting is needed. Here, we report a 3D bio-artificial muscle (BAM) bioengineered from green fluorescent protein-transduced C3H murine myoblasts as a novel in vitro tissue-based model for muscle innervation studies. Our cell biological and molecular analysis showed that this BAM is structurally similar to in vivo muscle tissue and can reach the perinatal differentiation stage, higher than does 2D culture. Effective clustering and morphological maturation of AchRs on BAMs induced by agrin and laminin indicate the functional activity and plasticity of this BAM system toward innervation. Taken together, our results show that the BAM provides a favorable 3D environment that at least partially recapitulates real physiological skeletal muscle with regard to innervation. With a convenience of fabrication and manipulation, this 3D in vitro system offers a novel model for studying mechanisms underlying skeletal muscle innervation and testing therapeutic strategies for relevant nervous and muscular diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.