ArticlePlant physiology2013
A cyclic nucleotide-gated channel (CNGC16) in pollen is critical for stress tolerance in pollen reproductive development.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed, 175 citations in OpenAlex.
- A BRI1-CNGC12 phosphorylation module links hormone signaling to manganese homeostasis in plants.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Anther and pollen thermotolerance: molecular mechanisms and implications for plant reproduction under heat stress.Planta · 2026Review
- Molecular Networks Governing Plant Responses to Heat and Cold Stress.Plants (Basel, Switzerland) · 2025Review
- Heat-stable protein PGSL1 enhances pollen germination and tube growth at high temperature.Nature communications · 2025Article
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- Infrared laser-induced gene expression in single cells characterized by quantitative imaging in Physcomitrium patens.Communications biology · 2024Article
- Understanding the mechanobiology of phytoacoustics through molecular Lens: Mechanisms and future perspectives.Journal of advanced research · 2024Review
- Genome Analysis ofInternational journal of molecular sciences · 2024Article
- Molecular mechanisms underlying the negative effects of transient heatwaves on crop fertility.Plant communications · 2024Review
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- Genome-Wide Exploration and Expression Analysis of the CNGC Gene Family in Eggplant (International journal of molecular sciences · 2023Article
- Identification and Expression of the CorA/MRS2/ALR Type Magnesium Transporters in Tomato.Plants (Basel, Switzerland) · 2023Article
- The Regulatory Network of Sweet Corn (International journal of molecular sciences · 2023Article
- Genome-wide identification and expression analysis of the cyclic nucleotide-gated ion channel (CNGC) gene family in Saccharum spontaneum.BMC genomics · 2023Article
- Overexpressing Vitamin C Defective 2 reduces fertility and alters Ca2+ signals in Arabidopsis pollen.Plant physiology · 2023Article
- Identification ofBiology · 2023Article
- Genome-Wide Analysis and Expression ofPlants (Basel, Switzerland) · 2023Article
- Multiple cyclic nucleotide-gated channels function as ABA-activated Ca2+ channels required for ABA-induced stomatal closure in Arabidopsis.The Plant cell · 2023Article
19 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 5 institutions in 4 countries.
Funding
Abstract
Cyclic nucleotide-gated channels (CNGCs) have been implicated in diverse aspects of plant growth and development, including responses to biotic and abiotic stress, as well as pollen tube growth and fertility. Here, genetic evidence identifies CNGC16 in Arabidopsis (Arabidopsis thaliana) as critical for pollen fertility under conditions of heat stress and drought. Two independent transfer DNA disruptions of cngc16 resulted in a greater than 10-fold stress-dependent reduction in pollen fitness and seed set. This phenotype was fully rescued through pollen expression of a CNGC16 transgene, indicating that cngc16-1 and 16-2 were both loss-of-function null alleles. The most stress-sensitive period for cngc16 pollen was during germination and the initiation of pollen tube tip growth. Pollen viability assays indicate that mutant pollen are also hypersensitive to external calcium chloride, a phenomenon analogous to calcium chloride hypersensitivities observed in other cngc mutants. A heat stress was found to increase concentrations of 3',5'-cyclic guanyl monophosphate in both pollen and leaves, as detected using an antibody-binding assay. A quantitative PCR analysis indicates that cngc16 mutant pollen have attenuated expression of several heat-stress response genes, including two heat shock transcription factor genes, HsfA2 and HsfB1. Together, these results provide evidence for a heat stress response pathway in pollen that connects a cyclic nucleotide signal, a Ca(2+)-permeable ion channel, and a signaling network that activates a downstream transcriptional heat shock response.
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