ArticleJournal of biomedical optics2013
Label-free photoacoustic microscopy of cytochromes.
Article in Journal of biomedical optics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
55 citing papers in PubMed, 122 citations in OpenAlex.
- Revealing hidden tissue architecture with mid-infrared dichroism photoacoustic microscopy.Light, science & applications · 2026Article
- Label-Free 3D Photoacoustic Imaging of Tumor Organoids for Volumetric Drug Screening.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Optoacoustic lenses for lateral sub-optical resolution elasticity imaging.Photoacoustics · 2025Article
- Optical-resolution parallel ultraviolet photoacoustic microscopy for slide-free histology.Science advances · 2024Article
- Resolution Enhancement Strategies in Photoacoustic Microscopy: A Comprehensive Review.Micromachines · 2024Review
- Controlling the sound of light: photoswitching optoacoustic imaging.Nature methods · 2024Review
- Metabolic light absorption, scattering, and emission (MetaLASE) microscopy.Science advances · 2024Article
- Quantitative Optical Imaging of Oxygen in Brain Vasculature.The journal of physical chemistry. B · 2024Review
- Virtual formalin-fixed and paraffin-embedded staining of fresh brain tissue via stimulated Raman CycleGAN model.Science advances · 2024Article
- Deep learning-enabled realistic virtual histology with ultraviolet photoacoustic remote sensing microscopy.Nature communications · 2023Article
- The sound of blood: photoacoustic imaging in blood analysis.Medicine in novel technology and devices · 2023Article
- Label-free intraoperative histology of bone tissue via deep-learning-assisted ultraviolet photoacoustic microscopy.Nature biomedical engineering · 2023Article
- Optical-resolution photoacoustic microscopy with a needle-shaped beam.Nature photonics · 2023Article
- Virtual histological staining of label-free total absorption photoacoustic remote sensing (TA-PARS).Scientific reports · 2022Article
- High-speed optical resolution photoacoustic microscopy with MEMS scanner using a novel and simple distortion correction method.Scientific reports · 2022Article
- Assessment of Nanoparticle-Mediated Tumor Oxygen Modulation by Photoacoustic Imaging.Biosensors · 2022Review
- Transient absorption spectroscopy and imaging of redox in muscle mitochondria.Biomedical optics express · 2022Article
- Multimodal 3D photoacoustic remote sensing and confocal fluorescence microscopy imaging.Journal of biomedical optics · 2021Article
- Photoacoustic Neuroimaging - Perspectives on a Maturing Imaging Technique and its Applications in Neuroscience.Frontiers in neuroscience · 2021Review
- Recent advances in photoacoustic tomography.BME frontiers · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Photoacoustic microscopy (PAM) has achieved submicron lateral resolution in showing subcellular structures; however, relatively few endogenous subcellular contrasts have so far been imaged. Given that the hemeprotein, mostly cytochromes in general cells, is optically absorbing around the Soret peak (~420 nm), we implemented label-free PAM of cytochromes in cytoplasm for the first time. By measuring the photoacoustic spectra of the oxidized and reduced states of fibroblast lysate and fitting the difference spectrum with three types of cytochromes, we found that the three cytochromes account for more than half the optical absorption in the cell lysate at 420 nm wavelength. Fixed fibroblasts on slides were imaged by PAM at 422 and 250 nm wavelengths to reveal cytoplasms and nuclei, respectively, as confirmed by standard staining histology. PAM was also applied to label-free histology of mouse ear sections by showing cytoplasms and nuclei of various cells. PAM of cytochromes in cytoplasm is expected to be a high-throughput, label-free technique for studying live cell functions, which cannot be accomplished by conventional histology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.