ArticleJournal of neuroscience methods2013
A time and cost efficient approach to functional and structural assessment of living neuronal tissue.
Article in Journal of neuroscience methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- AAV-BR1 targets endothelial cells in the retina to reveal their morphological diversity and to deliver Cx43.The Journal of comparative neurology · 2022Article
- Optogenetic Stimulation of Cholinergic Amacrine Cells Improves Capillary Blood Flow in Diabetic Retinopathy.Investigative ophthalmology & visual science · 2020Article
- Mouse models of X-linked juvenile retinoschisis have an early onset phenotype, the severity of which varies with genotype.Human molecular genetics · 2019Article
- Atypical Expression and Activation of GluN2A- and GluN2B-Containing NMDA Receptors at Ganglion Cells during Retinal Degeneration.Neuroscience · 2018Article
- Pannexin 1 sustains the electrophysiological responsiveness of retinal ganglion cells.Scientific reports · 2018Article
- Closed-Loop Efficient Searching of Optimal Electrical Stimulation Parameters for Preferential Excitation of Retinal Ganglion Cells.Frontiers in neuroscience · 2018Article
- Vascular Pericyte Impairment and Connexin43 Gap Junction Deficit Contribute to Vasomotor Decline in Diabetic Retinopathy.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- High-amplitude electrical stimulation can reduce elicited neuronal activity in visual prosthesis.Scientific reports · 2017Article
- Aberrant activity in retinal degeneration impairs central visual processing and relies on Cx36-containing gap junctions.Experimental eye research · 2016Article
- Leveraging Optogenetic-Based Neurovascular Circuit Characterization for Repair.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2016Review
- Increased phosphorylation of Cx36 gap junctions in the AII amacrine cells of RD retina.Frontiers in cellular neuroscience · 2015Article
- Intersublaminar vascular plexus: the correlation of retinal blood vessels with functional sublaminae of the inner plexiform layer.Investigative ophthalmology & visual science · 2014Article
- Optimized protocol for retinal wholemount preparation for imaging and immunohistochemistry.Journal of visualized experiments : JoVE · 2013Article
- Block of gap junctions eliminates aberrant activity and restores light responses during retinal degeneration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Correlated spontaneous activity persists in adult retina and is suppressed by inhibitory inputs.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
In this manuscript, we describe a protocol for capturing both physiological and structural properties of living neuronal tissue. An essential aspect of this method is its flexibility and fast turnaround time. It is a streamlined process that includes recording of electrophysiological neuronal activity, calcium imaging, and structural analysis. This is accomplished by placing intact tissue on a modified Millicell Biopore insert. The Biopore membrane suspends the tissue in the perfusion solution, allowing for complete access to nutrients, oxygen, and pharmacological agents. The ring that holds the membrane ensures its structural stability; forceps can be used to grip the ring without contacting the filter or the tissue, for easy transfer between multiple setups. We show that tissue readily adheres to the surface of the membrane, its entire surface is visible in transmitted light and accessible for recording and imaging, and remains responsive to physiological stimuli for extended periods of time.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.