ArticleAllergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology2013
Functional analysis of the impact of ORMDL3 expression on inflammation and activation of the unfolded protein response in human airway epithelial cells.
Article in Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 46 citations in OpenAlex.
- ORMDL Proteins Turnover via Proteasome and Autophagy Is Cell-Type Dependent and Tied to Ceramide Homeostasis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Orm2 promotes nitrogen-induced sphingolipid production and endocytosis via Orm1 phosphorylation.Journal of lipid research · 2025Article
- Heavy Metal Exposure-Mediated Dysregulation of Sphingolipid Metabolism.Antioxidants (Basel, Switzerland) · 2024Article
- ORMDL3 regulates NLRP3 inflammasome activation by maintaining ER-mitochondria contacts in human macrophages and dictates ulcerative colitis patient outcome.The Journal of biological chemistry · 2024Article
- "Stick a Fork in Me; I'm Done": Epithelial Cell Expression of ORMDL Sphingolipid Biosynthesis Regulator 3 Mediates Autophagic Cell Death.American journal of respiratory cell and molecular biology · 2022Article
- Role of unfolded proteins in lung disease.Thorax · 2021Review
- Translational Analysis of Moderate to Severe Asthma GWAS Signals Into Candidate Causal Genes and Their Functional, Tissue-Dependent and Disease-Related Associations.Frontiers in allergy · 2021Article
- ORMDL3 Functions as a Negative Regulator of Antigen-Mediated Mast Cell ActivationFrontiers in immunology · 2021Article
- Differential Expression of Ormdl Genes in the Islets of Mice and Humans with Obesity.iScience · 2020Article
- Update on the role of endoplasmic reticulum stress in asthma.American journal of translational research · 2020Review
- ORMDL3 and Asthma: Linking Sphingolipid Regulation to Altered T Cell Function.Frontiers in immunology · 2020Review
- Article
- The ORMDL3 asthma susceptibility gene regulates systemic ceramide levels without altering key asthma features in mice.The Journal of allergy and clinical immunology · 2019Article
- Investigating the regulatory role of ORMDL3 in airway barrier dysfunction using in vivo and in vitro models.International journal of molecular medicine · 2019Article
- Conjugated bile acids attenuate allergen-induced airway inflammation and hyperresponsiveness by inhibiting UPR transducers.JCI insight · 2019Article
- The Role of Autophagy and Related MicroRNAs in Inflammatory Bowel Disease.Gastroenterology research and practice · 2018Review
- Article
- Oxidative and endoplasmic reticulum stress in respiratory disease.Clinical & translational immunology · 2018Review
- Environment Changes Genetic Effects on Respiratory Conditions and Allergic Phenotypes.Scientific reports · 2017Article
- Endoplasmic reticulum stress in lung disease.European respiratory review : an official journal of the European Respiratory Society · 2017Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe gene ORMDL3 was shown to be associated with early-onset asthma susceptibility in multiple independent genome-wide and candidate-gene association studies. Asthmatic patients have elevated expression levels of this gene. ORMDL3 encodes a transmembrane protein localized in the endoplasmic reticulum (ER) that may be involved in ER stress and inflammation. It is essential to validate the genetic associations linking ORMDL3 with asthma through functional studies that confirm the biological relevance of this gene in disease. We investigated the effects of manipulating ORMDL3 expression levels in vitro in airway cells on innate immune inflammatory responses, ER stress and activation of the unfolded protein response (UPR).
methodsORMDL3 expression levels were manipulated in airway cells using an overexpression plasmid and siRNA technologies. Successful modulation of ORMDL3 was confirmed at both the gene and protein level. The functional impact of modulation of ORMDL3 expression levels on inflammatory responses and activation of the UPR were quantified using complementary cellular and molecular immunology techniques.
resultsCells with altered ORMDL3 levels responded equally well to innate immune stimuli and produced similar levels of pro-inflammatory cytokines compared to wild-type cells. Treatment with ER stress inducers, thapsigargin and tunicamycin, resulted in activation of the unfolded protein response (UPR). However, we observed no difference in UPR activation in cells with ORMDL3 knockdown compared to cells with normal ORMDL3 levels.
conclusionsOur results suggest that ORMDL3 variation in the airway epithelium is unlikely to play a significant role in modulating innate immune responses and the UPR in the lung.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.