ArticlePloS one2013
Transcriptome exploration in Leymus chinensis under saline-alkaline treatment using 454 pyrosequencing.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Identification of LsPROG gene in Leymus secalinus and validation of its function in Arabidopsis thaliana.BMC genomics · 2026Article
- Unambiguous chromosome identification by multicolour fluorescence in situ hybridisation reveals the genome organisation and evolution of sheepgrass (Leymus chinensis).BMC plant biology · 2026Article
- Integrated Transcriptome and Metabolome Analysis Identifies Key Genes Regulating Maize Tolerance to Alkaline Stress.International journal of molecular sciences · 2025Article
- Enhancing Plant Stress Tolerance: The Role ofInternational journal of molecular sciences · 2024Article
- Physiological and comparative transcriptome analysis of the response and adaptation mechanism of the photosynthetic function of mulberry (Plant signaling & behavior · 2022Article
- Understanding Omics Driven Plant Improvement andFrontiers in genetics · 2021Review
- Identification of a Major QTL and Candidate Gene Analysis of Salt Tolerance at the Bud Burst Stage in Rice (Oryza sativa L.) Using QTL-Seq and RNA-Seq.Rice (New York, N.Y.) · 2020Article
- A MYB-related transcription factor from sheepgrass, LcMYB2, promotes seed germination and root growth under drought stress.BMC plant biology · 2019Article
- bHLH92 from sheepgrass acts as a negative regulator of anthocyanin/proanthocyandin accumulation and influences seed dormancy.Journal of experimental botany · 2019Article
- Advances in Understanding the Physiological and Molecular Responses of Sugar Beet to Salt Stress.Frontiers in plant science · 2019Review
- Unigene-based RNA-seq provides insights on drought stress responses in Marsdenia tenacissima.PloS one · 2018Article
- Genome-Wide Analysis ofFrontiers in plant science · 2018Article
- Transcriptome profiling reveals the genetic basis of alkalinity tolerance in wheat.BMC genomics · 2017Article
- Proteome dynamics and physiological responses to short-term salt stress in Leymus chinensis leaves.PloS one · 2017Article
- Transcriptome analysis of genes involved in defense against alkaline stress in roots of wild jujube (Ziziphus acidojujuba).PloS one · 2017Article
- De novo transcriptome sequencing of Acer palmatum and comprehensive analysis of differentially expressed genes under salt stress in two contrasting genotypes.Molecular genetics and genomics : MGG · 2016Article
- De Novo Transcriptional Analysis of Alfalfa in Response to Saline-Alkaline Stress.Frontiers in plant science · 2016Article
- New Insights on Drought Stress Response by Global Investigation of Gene Expression Changes in Sheepgrass (Leymus chinensis).Frontiers in plant science · 2016Article
- De Novo Sequencing and Analysis of the Safflower Transcriptome to Discover Putative Genes Associated with Safflor Yellow in Carthamus tinctorius L.International journal of molecular sciences · 2015Article
- Prediction of Leymus arenarius (L.) antimicrobial peptides based on de novo transcriptome assembly.Plant molecular biology · 2015Article
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12 authors.
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Abstract
backgroundLeymus chinensis (Trin.) Tzvel. is a high saline-alkaline tolerant forage grass genus of the tribe Gramineae family, which also plays an important role in protection of natural environment. To date, little is known about the saline-alkaline tolerance of L. chinensis on the molecular level. To better understand the molecular mechanism of saline-alkaline tolerance in L. chinensis, 454 pyrosequencing was used for the transcriptome study.
resultsWe used Roche-454 massive parallel pyrosequencing technology to sequence two different cDNA libraries that were built from the two samples of control and under saline-alkaline treatment (optimal stress concentration-Hoagland solution with 100 mM NaCl and 200 mM NaHCO(3)). A total of 363,734 reads in control group and 526,267 reads in treatment group with an average length of 489 bp and 493 bp were obtained, respectively. The reads were assembled into 104,105 unigenes with MIRA sequence assemable software, among which, 73,665 unigenes were in control group, 88,016 unigenes in treatment group and 57,576 unigenes in both groups. According to the comparative expression analysis between the two groups with the threshold of "log2 Ratio ≥1", there were 36,497 up-regulated unegenes and 18,218 down-regulated unigenes predicted to be the differentially expressed genes. After gene annotation and pathway enrichment analysis, most of them were involved in stress and tolerant function, signal transduction, energy production and conversion, and inorganic ion transport. Furthermore, 16 of these differentially expressed genes were selected for real-time PCR validation, and they were successfully confirmed with the results of 454 pyrosequencing.
conclusionsThis work is the first time to study the transcriptome of L. chinensis under saline-alkaline treatment based on the 454-FLX massively parallel DNA sequencing platform. It also deepened studies on molecular mechanisms of saline-alkaline in L. chinensis, and constituted a database for future studies.
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