ArticleJournal of experimental botany2013
Virus-induced gene silencing of Arabidopsis thaliana gene homologues in wheat identifies genes conferring improved drought tolerance.
Article in Journal of experimental botany, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 93 citations in OpenAlex.
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- Efficient gene editing of a model fern species through gametophyte-based transformation.Plant physiology · 2024Article
- A review on strategies for crop improvement against drought stress through molecular insights.3 Biotech · 2024Review
- Genomic approaches to enhance adaptive plasticity to cope with soil constraints amidst climate change in wheat.The plant genome · 2024Review
- Polyploidization: A Biological Force That Enhances Stress Resistance.International journal of molecular sciences · 2024Review
- Endoplasmic reticulum stress-responsive microRNAs are involved in the regulation of abiotic stresses in wheat.Plant cell reports · 2023Article
- Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance.Journal of Zhejiang University. Science. B · 2023Review
- CRISPR-Cas9 Gene Editing of thePlants (Basel, Switzerland) · 2022Article
- Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops.Heredity · 2022Article
- Genome Editing Targets for Improving Nutrient Use Efficiency and Nutrient Stress Adaptation.Frontiers in genetics · 2022Review
- Progressive Genomic Approaches to Explore Drought- and Salt-Induced Oxidative Stress Responses in Plants under Changing Climate.Plants (Basel, Switzerland) · 2021Review
- Protein Farnesylation Takes Part in Arabidopsis Seed Development.Frontiers in plant science · 2021Article
- Meiotic crossover reduction by virus-induced gene silencing enables the efficient generation of chromosome substitution lines and reverse breeding in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2020Article
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5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In a non-model staple crop like wheat (Triticum aestivumI L.), functional validation of potential drought stress responsive genes identified in Arabidopsis could provide gene targets for breeding. Virus-induced gene silencing (VIGS) of genes of interest can overcome the inherent problems of polyploidy and limited transformation potential that hamper functional validation studies in wheat. In this study, three potential candidate genes shown to be involved in abiotic stress response pathways in Arabidopsis thaliana were selected for VIGS experiments in wheat. These include Era1 (enhanced response to abscisic acid), Cyp707a (ABA 8'-hydroxylase), and Sal1 (inositol polyphosphate 1-phosphatase). Gene homologues for these three genes were identified in wheat and cloned in the viral vector barley stripe mosaic virus (BSMV) in the antisense direction, followed by rub inoculation of BSMV viral RNA transcripts onto wheat plants. Quantitative real-time PCR showed that VIGS-treated wheat plants had significant reductions in target gene transcripts. When VIGS-treated plants generated for Era1 and Sal1 were subjected to limiting water conditions, they showed increased relative water content, improved water use efficiency, reduced gas exchange, and better vigour compared to water-stressed control plants inoculated with RNA from the empty viral vector (BSMV0). In comparison, the Cyp707a-silenced plants showed no improvement over BSMV0-inoculated plants under limited water condition. These results indicate that Era1 and Sal1 play important roles in conferring drought tolerance in wheat. Other traits affected by Era1 silencing were also studied. Delayed seed germination in Era1-silenced plants suggests this gene may be a useful target for developing resistance to pre-harvest sprouting.
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