ArticleThe Journal of biological chemistry2013
Myogenin recruits the histone chaperone facilitates chromatin transcription (FACT) to promote nucleosome disassembly at muscle-specific genes.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 31 citations in OpenAlex.
- Genome-Wide Association Study of Vertical Jump Performance Among Elite Badminton Players.International journal of molecular sciences · 2026Article
- Facilitates Chromatin Transcription in Breast and Other Cancers.Advances in experimental medicine and biology · 2024Review
- Lysine methylation signaling in skeletal muscle biology: from myogenesis to clinical insights.The Biochemical journal · 2023Review
- Transcriptomic Analysis Reveals mRNA and Alternative Splicing Events in Ovine Skeletal Muscle Satellite Cells during Proliferation and Differentiation.Animals : an open access journal from MDPI · 2023Article
- A topographic atlas defines developmental origins of cell heterogeneity in the human embryonic lung.Nature cell biology · 2023Article
- Injectable Bacteria-Sensitive Hydrogel Promotes Repair of Infected Fractures via Sustained Release of miRNA Antagonist.ACS applied materials & interfaces · 2022Article
- Proteasomal Regulation of Mammalian SPT16 in Controlling Transcription.Molecular and cellular biology · 2021Article
- Myogenin is required for assembly of the transcription machinery on muscle genes during skeletal muscle differentiation.PloS one · 2021Article
- Congenital Splay Leg Syndrome in Piglets-Current Knowledge and a New Approach to Etiology.Frontiers in veterinary science · 2021Review
- The role of FACT in managing chromatin: disruption, assembly, or repair?Nucleic acids research · 2020Review
- Transcriptomic Profile of Primary Culture of Skeletal Muscle Cells Isolated from Semitendinosus Muscle of Beef and Dairy Bulls.International journal of molecular sciences · 2020Article
- Histone chaperone FACT is essential to overcome replication stress in mammalian cells.Oncogene · 2020Article
- SSRP1 promotes colorectal cancer progression and is negatively regulated by miR-28-5p.Journal of cellular and molecular medicine · 2019Article
- Histone Chaperone FACT and Curaxins: Effects on Genome Structure and Function.Journal of cancer metastasis and treatment · 2019Article
- FACT complex gene duplicates exhibit redundant and non-redundant functions in C. elegans.Developmental biology · 2018Article
- FACT Inhibition Blocks Induction But Not Maintenance of Pluripotency.Stem cells and development · 2018Article
- Structure and function of the histone chaperone FACT - Resolving FACTual issues.Biochimica et biophysica acta. Gene regulatory mechanisms · 2018Review
- Temporal regulation of chromatin during myoblast differentiation.Seminars in cell & developmental biology · 2017Review
- Level of FACT defines the transcriptional landscape and aggressive phenotype of breast cancer cells.Oncotarget · 2017Article
- Pluripotency transcription factor Oct4 mediates stepwise nucleosome demethylation and depletion.Molecular and cellular biology · 2015Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Facilitates chromatin transcription (FACT) functions to reorganize nucleosomes by acting as a histone chaperone that destabilizes and restores nucleosomal structure. The FACT complex is composed of two subunits: SSRP1 and SPT16. We have discovered that myogenin interacts with the FACT complex. Transfection of FACT subunits with myogenin is highly stimulatory for endogenous muscle gene expression in 10T1/2 cells. We have also found that FACT subunits do not associate with differentiation-specific genes while C2C12 cells are proliferating but are recruited to muscle-specific genes as differentiation initiates and then dissociate as differentiation proceeds. The recruitment is dependent on myogenin, as knockdowns of myogenin show no recruitment of the FACT complex. These data suggest that FACT is involved in the early steps of gene activation through its histone chaperone activities that serve to open the chromatin structure and facilitate transcription. Consistent with this hypothesis, we find that nucleosomes are depleted at muscle-specific promoters upon differentiation and that this activity is dependent on the presence of FACT. Our results show that the FACT complex promotes myogenin-dependent transcription and suggest that FACT plays an important role in the establishment of the appropriate transcription profile in a differentiated muscle cell.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.