ArticleAmerican journal of physiology. Regulatory, integrative and comparative physiology2013
The tight junction protein claudin-b regulates epithelial permeability and sodium handling in larval zebrafish, Danio rerio.
Article in American journal of physiology. Regulatory, integrative and comparative physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 54 citations in OpenAlex.
- Whole-body single-cell atlas of an adult vertebrate in homeostasis and regeneration.bioRxiv : the preprint server for biology · 2026Article
- Injury-induced electrochemical coupling triggers organ growth.Science advances · 2026Article
- A novel gene-based model for prognosis prediction of head and neck squamous cell carcinoma.Heliyon · 2024Article
- A survey of water chemistry used in zebrafish facilities and their effects on early zebrafish development.F1000Research · 2024Article
- Article
- EGF-mediated suppression of cell extrusion during mucosal damage attenuates opportunistic fungal invasion.Cell reports · 2021Article
- Drinking and Water Handling in the Medaka Intestine: A Possible Role of Claudin-15 in Paracellular Absorption?International journal of molecular sciences · 2020Article
- Physiological protective action of dissolved organic carbon on ion regulation and nitrogenous waste excretion of zebrafish (Danio rerio) exposed to low pH in ion-poor water.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2018Article
- Zebrafish as a Model System for Investigating the Compensatory Regulation of Ionic Balance during Metabolic Acidosis.International journal of molecular sciences · 2018Review
- Protease signaling regulates apical cell extrusion, cell contacts, and proliferation in epithelia.The Journal of cell biology · 2018Article
- A study of the damage of the intestinal mucosa barrier structure and function of Ctenopharyngodon idella with Aeromonas hydrophila.Fish physiology and biochemistry · 2017Article
- Nuclear Localized LSR: A Novel Regulator of Breast Cancer Behavior and Tumorigenesis.Molecular cancer research : MCR · 2017Article
- Article
- Tracing the dynamics of gene transcripts after organismal death.Open biology · 2017Article
- A role for sodium-chloride cotransporters in the rapid regulation of ion uptake following acute environmental acidosis: new insights from the zebrafish model.American journal of physiology. Cell physiology · 2016Article
- The response of claudin-like transmembrane septate junction proteins to altered environmental ion levels in the larval mosquito Aedes aegypti.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2016Article
- The basal function of teleost prolactin as a key regulator on ion uptake identified with zebrafish knockout models.Scientific reports · 2016Article
- Pulmonary epithelial barrier function: some new players and mechanisms.American journal of physiology. Lung cellular and molecular physiology · 2015Review
- Involvement of the calcium-sensing receptor in calcium homeostasis in larval zebrafish exposed to low environmental calcium.American journal of physiology. Regulatory, integrative and comparative physiology · 2014Article
- The role of aquaporin and tight junction proteins in the regulation of water movement in larval zebrafish (Danio rerio).PloS one · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The functional role of the tight junction protein claudin-b in larval zebrafish (Danio rerio) was investigated. We showed that claudin-b protein is expressed at epithelial cell-cell contacts on the skin. Translational gene knockdown of claudin-b protein expression caused developmental defects, including edema in the pericardial cavity and yolk sac. Claudin-b morphants exhibited an increase in epithelial permeability to the paracellular marker polyethylene glycol (PEG-4000) and fluorescein isothiocyanate-dextran (FD-4). Accumulation of FD-4 was confined mainly to the yolk sac and pericardial cavity in the claudin-b morphants, suggesting these regions became particularly leaky in the absence of claudin-b expression. Additionally, Na(+) efflux was substantially increased in the claudin-b morphants, which contributed to a significant reduction in whole-body Na(+) levels. These results indicate that claudin-b normally acts as a paracellular barrier to Na(+). Nevertheless, the elevated loss of Na(+) in the morphants was compensated by an increase in Na(+) uptake. Notably, we observed that the increased Na(+) uptake in the morphants was attenuated in the presence of the selective Na(+)/Cl(-)-cotransporter (NCC) inhibitor metolazone, or during exposure to Cl(-)-free water. These results suggested that the increased Na(+) uptake in the morphants was, at least in part, mediated by NCC. Furthermore, treatment with an H(+)-ATPase inhibitor bafilomycin A1 was found to reduce Na(+) uptake in the morphants, suggesting that H(+)-ATPase activity was essential to provide a driving force for Na(+) uptake. Overall, the results suggest that claudin-b plays an important role in regulating epithelial permeability and Na(+) handling in zebrafish.
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