ArticleMolecular biology of the cell2013
Rap1 and Canoe/afadin are essential for establishment of apical-basal polarity in the Drosophila embryo.
Article in Molecular biology of the cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 89 citations in OpenAlex.
- The Rap1 GTPase performs two separable functions during cellularization to enable ventral furrow formation.bioRxiv : the preprint server for biology · 2026Article
- A key role of Canoe's intrinsically disordered region in linking cell junctions to the cytoskeleton.The Journal of cell biology · 2025Article
- PAK4 promotes vertex remodeling to maintain epithelial integrity and barrier function.bioRxiv : the preprint server for biology · 2025Article
- α-catenin phosphorylation is actomyosin-sensitive and required for epithelial barrier functions through Afadin.bioRxiv : the preprint server for biology · 2025Article
- Cytoplasmic localization of the mRNA encoding actin regulator, Serendipity-bioRxiv : the preprint server for biology · 2025Article
- Proximity proteomics provides a new resource for exploring the function of Afadin and the complexity of cell-cell adherens junctions.Biology open · 2025Article
- EGFR-dependent actomyosin patterning coordinates morphogenetic movements between tissues in Drosophila melanogaster.Developmental cell · 2025Article
- The dual Ras-association domains of Drosophila Canoe have differential roles in linking cell junctions to the cytoskeleton during morphogenesis.Journal of cell science · 2024Article
- Proximity proteomics provides a new resource for exploring the function of Afadin and the complexity of cell-cell adherens junctions.bioRxiv : the preprint server for biology · 2024Article
- C. elegans Afadin is required for epidermal morphogenesis and functionally interfaces with the cadherin-catenin complex and RhoGAP PAC-1/ARHGAP21.Developmental biology · 2024Article
- The Dilute domain in Canoe is not essential for linking cell junctions to the cytoskeleton but supports morphogenesis robustness.Journal of cell science · 2024Article
- Origin and development of primary animal epithelia.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- Kinesin-1 patterns Par-1 and Rho signaling at the cortex of syncytial embryos of Drosophila.The Journal of cell biology · 2024Article
- EGFR-dependent actomyosin patterning coordinates morphogenetic movements between tissues.bioRxiv : the preprint server for biology · 2023Article
- The Dilute domain of Canoe is not essential for Canoe's role in linking adherens junctions to the cytoskeleton but contributes to robustness of morphogenesis.bioRxiv : the preprint server for biology · 2023Article
- Rap1 regulates lumen continuity via Afadin in renal epithelia.Developmental biology · 2023Article
- Polychaetoid/ZO-1 strengthens cell junctions under tension while localizing differently than core adherens junction proteins.Molecular biology of the cell · 2023Article
- Zyxin contributes to coupling between cell junctions and contractile actomyosin networks during apical constriction.PLoS genetics · 2023Article
- Polychaetoid/ZO-1 strengthens cell junctions under tension while localizing differently than core adherens junction proteins.bioRxiv : the preprint server for biology · 2023Article
- Membrane-actin interactions in morphogenesis: Lessons learned from Drosophila cellularization.Seminars in cell & developmental biology · 2023Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The establishment and maintenance of apical-basal cell polarity is critical for assembling epithelia and maintaining organ architecture. Drosophila embryos provide a superb model. In the current view, apically positioned Bazooka/Par3 is the initial polarity cue as cells form during cellularization. Bazooka then helps to position both adherens junctions and atypical protein kinase C (aPKC). Although a polarized cytoskeleton is critical for Bazooka positioning, proteins mediating this remained unknown. We found that the small GTPase Rap1 and the actin-junctional linker Canoe/afadin are essential for polarity establishment, as both adherens junctions and Bazooka are mispositioned in their absence. Rap1 and Canoe do not simply organize the cytoskeleton, as actin and microtubules become properly polarized in their absence. Canoe can recruit Bazooka when ectopically expressed, but they do not obligatorily colocalize. Rap1 and Canoe play continuing roles in Bazooka localization during gastrulation, but other polarity cues partially restore apical Bazooka in the absence of Rap1 or Canoe. We next tested the current linear model for polarity establishment. Both Bazooka and aPKC regulate Canoe localization despite being "downstream" of Canoe. Further, Rap1, Bazooka, and aPKC, but not Canoe, regulate columnar cell shape. These data reshape our view, suggesting that polarity establishment is regulated by a protein network rather than a linear pathway.
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