ArticleBMC infectious diseases2013
Prospective European-wide multicentre study on a blood based real-time PCR for the diagnosis of acute schistosomiasis.
Article in BMC infectious diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 83 citations in OpenAlex.
- Diagnostic accuracy of plasma cell-free DNA qPCR for Schistosoma haematobium assessed by Bayesian latent class analysis in a cohort of pregnant women from Lambaréné, Gabon.Infectious diseases of poverty · 2026Article
- Hepatosplenic Schistosomiasis Presenting as Melena in an Adolescent Filipino Male: A Case Report and Literature Review.Acta medica Philippina · 2025Article
- Pediatric urogenital schistosomiasis diagnosed in France.Pediatric nephrology (Berlin, Germany) · 2024Article
- Diagnosis of Human Trematode Infections.Advances in experimental medicine and biology · 2024Review
- Review
- Advances in Diagnosis of Schistosomiasis: Focus on Challenges and Future Approaches.International journal of general medicine · 2023Review
- High prevalence of urinary schistosomiasis in a desert population: results from an exploratory study around the Ounianga lakes in Chad.Infectious diseases of poverty · 2022Article
- Article
- Low Sensitivity of Real Time PCRs Targeting Retrotransposon Sequences for the Detection ofPathogens (Basel, Switzerland) · 2021Article
- Serology- and Blood-PCR-Based Screening for Schistosomiasis in Pregnant Women in Madagascar-A Cross-Sectional Study and Test Comparison Approach.Pathogens (Basel, Switzerland) · 2021Article
- Evaluation of serum-based real-time PCR to detect Schistosoma mansoni infection before and after treatment.Infectious diseases of poverty · 2020Article
- Case Report of Urinary Schistosomiasis in a Returned Traveler in Korea.The Korean journal of parasitology · 2020Article
- A Real-Time PCR Assay for the Diagnosis of Intestinal Schistosomiasis and Cure Assessment After the Treatment of Individuals With Low Parasite Burden.Frontiers in immunology · 2020Article
- Real-time PCR for diagnosis of imported schistosomiasis.PLoS neglected tropical diseases · 2019Article
- Performance of an Ultra-Sensitive Assay Targeting the Circulating Anodic Antigen (CAA) for Detection ofFrontiers in immunology · 2019Article
- The Advances in Molecular and New Point-of-Care (POC) Diagnosis of Schistosomiasis Pre- and Post-praziquantel Use: In the Pursuit of More Reliable Approaches for Low Endemic and Non-endemic Areas.Frontiers in immunology · 2019Review
- Schistosoma mansoni infection and socio-behavioural predictors of HIV risk: a cross-sectional study in women from Uganda.BMC infectious diseases · 2018Article
- [Urogenital tuberculosis and schistosomiasis (bilharzia) : Urological challenges in displaced persons].Der Urologe. Ausg. A · 2018Review
- DNA Diagnostics for Schistosomiasis Control.Tropical medicine and infectious disease · 2018Review
- [Eosinophilia in pulmonary infections].Der Pneumologe · 2018Review
Corrections and comments
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Authors and funding
13 authors at 10 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAcute schistosomiasis constitutes a rare but serious condition in individuals experiencing their first prepatent Schistosoma infection. To circumvent costly and time-consuming diagnostics, an early and rapid diagnosis is required. So far, classic diagnostic tools such as parasite microscopy or serology lack considerable sensitivity at this early stage of Schistosoma infection. To validate the use of a blood based real-time polymerase chain reaction (PCR) test for the detection of Schistosoma DNA in patients with acute schistosomiasis who acquired their infection in various endemic regions we conducted a European-wide prospective study in 11 centres specialized in travel medicine and tropical medicine.
methodsPatients with a history of recent travelling to schistosomiasis endemic regions and freshwater contacts, an episode of fever (body temperature ≥38.5°C) and an absolute or relative eosinophil count of ≥700/μl or 10%, were eligible for participation. PCR testing with DNA extracted from serum was compared with results from serology and microscopy.
resultsOf the 38 patients with acute schistosomiasis included into the study, PCR detected Schistosoma DNA in 35 patients at initial presentation (sensitivity 92%). In contrast, sensitivity of serology (enzyme immunoassay and/or immunofluorescence assay) or parasite microscopy was only 70% and 24%, respectively.
conclusionFor the early diagnosis of acute schistosomiasis, real-time PCR for the detection of schistosoma DNA in serum is more sensitive than classic diagnostic tools such as serology or microscopy, irrespective of the region of infection. Generalization of the results to all Schistosoma species may be difficult as in the study presented here only eggs of S. mansoni were detected by microscopy. A minimum amount of two millilitre of serum is required for sufficient diagnostic accuracy.
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