ArticleMolecular pharmaceutics2013
Light-induced conversion of Trp to Gly and Gly hydroperoxide in IgG1.
Article in Molecular pharmaceutics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 43 citations in OpenAlex.
- Stability of Protein Pharmaceuticals: Recent Advances.Pharmaceutical research · 2024Review
- Identification of Hetero-aggregates in Antibody Co-formulations by Multi-dimensional Liquid Chromatography Coupled to Mass Spectrometry.Analytical chemistry · 2023Article
- Photo-Oxidation of Therapeutic Protein Formulations: From Radical Formation to Analytical Techniques.Pharmaceutics · 2021Review
- Effect of Addition of Tryptophan on Aggregation of Apo-α-Lactalbumin Induced by UV-Light.Foods (Basel, Switzerland) · 2021Article
- Microchip Electrophoresis Assay for Calmodulin Binding Proteins.Journal of separation science · 2021Article
- A synthetic mimic of phosphodiesterase type 5 based on corona phase molecular recognition of single-walled carbon nanotubes.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Photo-Degradation of Therapeutic Proteins: Mechanistic Aspects.Pharmaceutical research · 2020Review
- Thiyl Radical Reactions in the Chemical Degradation of Pharmaceutical Proteins.Molecules (Basel, Switzerland) · 2019Review
- Article
- Identification of D-Amino Acids in Light Exposed mAb Formulations.Pharmaceutical research · 2018Article
- Radical Anions of Oxidized vs. Reduced Oxytocin: Influence of Disulfide Bridges on CID and Vacuum UV Photo-Fragmentation.Journal of the American Society for Mass Spectrometry · 2018Article
- Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting.Angewandte Chemie (International ed. in English) · 2018Article
- Light-Induced Covalent Buffer Adducts to Histidine in a Model Protein.Pharmaceutical research · 2018Article
- Photodegradation Pathways of Protein Disulfides: Human Growth Hormone.Pharmaceutical research · 2017Article
- Photo-oxidation of IgG1 and Model Peptides: Detection and Analysis of Triply Oxidized His and Trp Side Chain Cleavage Products.Pharmaceutical research · 2017Article
- Comparative Evaluation of the Chemical Stability of 4 Well-Defined Immunoglobulin G1-Fc Glycoforms.Journal of pharmaceutical sciences · 2016Article
- Bond-specific dissociation following excitation energy transfer for distance constraint determination in the gas phase.Journal of the American Chemical Society · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The exposure of IgG1 in aqueous solution to light with λ = 254 nm or λ > 295 nm yields products consistent with Trp radical cation formation followed by (α)C-(β)C cleavage of the Trp side chain. The resulting glycyl radicals either are reduced to Gly or add oxygen prior to reduction to Gly hydroperoxide. Photoirradiation at λ = 254 nm targets Trp at positions 191 (light chain), 309 and 377 (heavy chain) while photoirradiation at λ > 295 nm targets Trp at position 309 (heavy chain). Mechanistically, the formation of Trp radical cations likely proceeds via photoinduced electron or hydrogen transfer to disulfide bonds, yielding thiyl radicals and thiols, where thiols may serve as reductants for the intermediary glycyl or glycylperoxyl radicals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.