ArticleACS chemical biology2013
Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
Article in ACS chemical biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 56 citations in OpenAlex.
- Leveraging Autonomous Biological Oscillators for Directed Evolution of Switchable Proteins.Advances in experimental medicine and biology · 2026Review
- Chemical Probes that Target a Dissociative LuxR-Type Quorum Sensing Receptor in Gram-Negative Bacteria.ACS chemical biology · 2025Article
- Developing Quorum Sensing-Based Collaborative Dynamic Control System in Halomonas TD01.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner.Genome biology · 2025Article
- Engineering quorum sensing-based genetic circuits enhances growth and productivity robustness of industrial E. coli at low pH.Microbial cell factories · 2024Article
- Delaying production with prokaryotic inducible expression systems.Microbial cell factories · 2024Review
- A synthetic population-level oscillator in non-microfluidic environments.Communications biology · 2023Article
- Engineering of Synthetic Transcriptional Switches in Yeast.Life (Basel, Switzerland) · 2022Review
- Robust and flexible platform for directed evolution of yeast genetic switches.Nature communications · 2021Article
- Synthetic microbial consortia for biosynthesis and biodegradation: promises and challenges.Journal of industrial microbiology & biotechnology · 2019Review
- Combining directed evolution of pathway enzymes and dynamic pathway regulation using a quorum-sensing circuit to improve the production of 4-hydroxyphenylacetic acid inBiotechnology for biofuels · 2019Article
- Tools for engineering coordinated system behaviour in synthetic microbial consortia.Nature communications · 2018Article
- Article
- Transcriptional control of motility enables directional movement of Escherichia coli in a signal gradient.Scientific reports · 2017Article
- Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit.Nature biotechnology · 2017Article
- Article
- Dynamic metabolic engineering: New strategies for developing responsive cell factories.Biotechnology journal · 2015Review
- Unraveling the contributions of hydrogen-bonding interactions to the activity of native and non-native ligands in the quorum-sensing receptor LasR.Organic & biomolecular chemistry · 2015Article
- Article
- Can the natural diversity of quorum-sensing advance synthetic biology?Frontiers in bioengineering and biotechnology · 2015Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The use of cell-cell communication or "quorum sensing (QS)" elements from Gram-negative Proteobacteria has enabled synthetic biologists to begin engineering systems composed of multiple interacting organisms. However, additional tools are necessary if we are to progress toward synthetic microbial consortia that exhibit more complex, dynamic behaviors. EsaR from Pantoea stewartii subsp. stewartii is a QS regulator that binds to DNA as an apoprotein and releases the DNA when it binds to its cognate signal molecule, 3-oxohexanoyl-homoserine lactone (3OC6HSL). In the absence of 3OC6HSL, EsaR binds to DNA and can act as either an activator or a repressor of transcription. Gene expression from P(esaR), which is repressed by wild-type EsaR, requires 100- to 1000-fold higher concentrations of signal than commonly used QS activators, such as LuxR and LasR. Here we have identified EsaR variants with increased sensitivity to 3OC6HSL using directed evolution and a dual ON/OFF screening strategy. Although we targeted EsaR-dependent derepression of P(esaR), our EsaR variants also showed increased 3OC6HSL sensitivity at a second promoter, P(esaS), which is activated by EsaR in the absence of 3OC6HSL. Here, the increase in AHL sensitivity led to gene expression being turned off at lower concentrations of 3OC6HSL. Overall, we have increased the signal sensitivity of EsaR more than 70-fold and generated a set of EsaR variants that recognize 3OC6HSL concentrations ranging over 4 orders of magnitude. QS-dependent transcriptional regulators that bind to DNA and are active in the absence of a QS signal represent a new set of tools for engineering cell-cell communication-dependent gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.