ArticleScientific reports2013
Chaperone-assisted thermostability engineering of a soluble T cell receptor using phage display.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Targeting cancer with precision: strategical insights into TCR-engineered T cell therapies.Theranostics · 2025Review
- FkpA enhances membrane protein folding using an extensive interaction surface.Protein science : a publication of the Protein Society · 2023Article
- Affinity maturation of TCR-like antibodies using phage display guided by structural modeling.Protein engineering, design & selection : PEDS · 2022Article
- The making of multivalent gamma delta TCR anti-CD3 bispecific T cell engagers.Frontiers in immunology · 2022Article
- Engineering soluble T-cell receptors for therapy.The FEBS journal · 2021Review
- A high-affinity human TCR-like antibody detects celiac disease gluten peptide-MHC complexes and inhibits T cell activation.Science immunology · 2021Article
- Identification of drug-specific public TCR driving severe cutaneous adverse reactions.Nature communications · 2019Article
- Human cytomegalovirus-specific T-cell receptor engineered for high affinity and soluble expression using mammalian cell display.The Journal of biological chemistry · 2019Article
- A humanized TCR retaining authentic specificity and affinity conferred potent anti-tumour cytotoxicity.Immunology · 2018Article
- Crystal structure of an L chain optimised 14F7 anti-ganglioside Fv suggests a unique tumour-specificity through an unusual H-chain CDR3 architecture.Scientific reports · 2018Article
- Soluble T-cell receptor design influences functional yield in an E. coli chaperone-assisted expression system.PloS one · 2018Article
- A TCRα framework-centered codon shapes a biased T cell repertoire through direct MHC and CDR3β interactions.JCI insight · 2017Article
- Multivalent pIX phage display selects for distinct and improved antibody properties.Scientific reports · 2016Article
- Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display.Frontiers in microbiology · 2016Review
- Increased Fab thermoresistance via VH-targeted directed evolution.Protein engineering, design & selection : PEDS · 2015Article
- Folding LacZ in the periplasm of Escherichia coli.Journal of bacteriology · 2014Article
- Phage Display Engineered T Cell Receptors as Tools for the Study of Tumor Peptide-MHC Interactions.Frontiers in oncology · 2014Review
- Review
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We here report a novel phage display selection strategy enabling fast and easy selection of thermostabilized proteins. The approach is illustrated with stabilization of an aggregation-prone soluble single chain T cell receptor (scTCR) characteristic of the murine MOPC315 myeloma model. Random mutation scTCR phage libraries were prepared in E. coli over-expressing the periplasmic chaperone FkpA, and such over-expression during library preparation proved crucial for successful downstream selection. The thermostabilized scTCR(mut) variants selected were produced in high yields and isolated as monomers. Thus, the purified scTCRs could be studied with regard to specificity and equilibrium binding kinetics to pMHC using surface plasmon resonance (SPR). The results demonstrate a difference in affinity for pMHCs that display germ line or tumor-specific peptides which explains the tumor-specific reactivity of the TCR. This FkpA-assisted thermostabilization strategy extends the utility of recombinant TCRs and furthermore, may be of general use for efficient evolution of proteins.
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