ArticleScientific reports2013
PAPTi: a peptide aptamer interference toolkit for perturbation of protein-protein interaction networks.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Studying Protein Function Using Nanobodies and Other Protein Binders in Drosophila.Methods in molecular biology (Clifton, N.J.) · 2022Article
- FN3-based monobodies selective for the receptor binding domain of the SARS-CoV-2 spike protein.New biotechnology · 2021Article
- Review
- Advances in targeted degradation of endogenous proteins.Cellular and molecular life sciences : CMLS · 2019Review
- Generating FN3-Based Affinity Reagents Through Phage Display.Current protocols in chemical biology · 2018Article
- Development of a novel peptide aptamer-based immunoassay to detect Zika virus in serum and urine.Theranostics · 2018Article
- Generation of specific inhibitors of SUMO-1- and SUMO-2/3-mediated protein-protein interactions using Affimer (Adhiron) technology.Science signaling · 2017Article
- Identification and characterization of a novel Sso7d scaffold-based binder against Notch1.Scientific reports · 2017Article
- Rapid Detection of Avian Influenza Virus by Fluorescent Diagnostic Assay using an Epitope-Derived Peptide.Theranostics · 2017Article
- Loss-of-function genetic tools for animal models: cross-species and cross-platform differences.Nature reviews. Genetics · 2017Review
- An E3-ligase-based method for ablating inhibitory synapses.Nature methods · 2016Article
- Prediction of the anti-inflammatory mechanisms of curcumin by module-based protein interaction network analysis.Acta pharmaceutica Sinica. B · 2015Article
- Synthetic biology for pharmaceutical drug discovery.Drug design, development and therapy · 2015Review
- Protein interference applications in cellular and developmental biology using DARPins that recognize GFP and mCherry.Biology open · 2014Article
- Wnt-Notch signalling crosstalk in development and disease.Cellular and molecular life sciences : CMLS · 2014Review
- Peptide aptamers define distinct EB1- and EB3-binding motifs and interfere with microtubule dynamics.Molecular biology of the cell · 2014Article
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Authors and funding
5 authors.
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Abstract
Signaling proteins often form dynamic protein-protein interaction (PPI) complexes to achieve multi-functionality. Methods to abrogate a subset of PPI interfaces without depleting the full-length protein will be valuable for structure-function relationship annotations. Here, we describe the use of Peptide Aptamer Interference (PAPTi) approach for structure-function network studies. We identified peptide aptamers against Dishevelled (Dsh) and β-catenin (β-cat) to target the Wnt signaling pathway and demonstrate that these FN3-based MONOBODYs (FNDYs) can be used to perturb protein activities both in vitro and in vivo. Further, to investigate the crosstalk between the Wnt and Notch pathways, we isolated FNDYs against the Notch Ankyrin (ANK) region and demonstrate that perturbing the ANK domain of Notch increases the inhibitory activity of Notch towards Wnt signaling. Altogether, these studies demonstrate the power of the PAPTi approach to dissect specific PPI interactions within signaling networks.
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