ArticleJournal of gastroenterology2013
Ets1 and heat shock factor 1 regulate transcription of the Transformer 2β gene in human colon cancer cells.
Article in Journal of gastroenterology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 24 citations in OpenAlex.
- HSF1 remodels mitochondrial biogenesis and function in cancer cells via TIMM17A.bioRxiv : the preprint server for biology · 2025Article
- Antisense oligonucleotide-mediated TRA2β poison exon inclusion induces the expression of a lncRNA with anti-tumor effects.Nature communications · 2025Article
- Functional transcriptome analysis revealed major changes in pathways affecting systems biology of Tharparkar cattle under seasonal heat stress.3 Biotech · 2024Article
- Serpine1 mRNA confers mesenchymal characteristics to the cell and promotes CD8+ T cells exclusion from colon adenocarcinomas.Cell death discovery · 2024Article
- Review
- A common intronic single nucleotide variant modifies PKD1 expression level.Clinical genetics · 2022Article
- Overexpression of the transcribed ultraconserved region Uc.138 accelerates colon cancer progression.Scientific reports · 2021Article
- The Multifaceted Role of HSF1 in Tumorigenesis.Advances in experimental medicine and biology · 2020Review
- HnRNPA1 interacts with G-quadruplex in the TRA2B promoter and stimulates its transcription in human colon cancer cells.Scientific reports · 2019Article
- Alternative-splicing defects in cancer: Splicing regulators and their downstream targets, guiding the way to novel cancer therapeutics.Wiley interdisciplinary reviews. RNA · 2018Review
- miR-335 inhibited cell proliferation of lung cancer cells by target Tra2β.Cancer science · 2018Article
- Small molecule modulation of splicing factor expression is associated with rescue from cellular senescence.BMC cell biology · 2017Article
- The Arabidopsis SR45 Splicing Factor, a Negative Regulator of Sugar Signaling, Modulates SNF1-Related Protein Kinase 1 Stability.The Plant cell · 2016Article
- Ultraconserved region-containing Transformer 2β4 controls senescence of colon cancer cells.Oncogenesis · 2016Article
- Multifaceted roles of HSF1 in cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Review
- Transformer 2β and miR-204 regulate apoptosis through competitive binding to 3' UTR of BCL2 mRNA.Cell death and differentiation · 2015Article
- Prognostic value of transformer 2β expression in prostate cancer.International journal of clinical and experimental pathology · 2015Article
- Transformer 2β (Tra2β/SFRS10) positively regulates the progression of NSCLC via promoting cell proliferation.Journal of molecular histology · 2014Article
- HuR regulates alternative splicing of the TRA2β gene in human colon cancer cells under oxidative stress.Molecular and cellular biology · 2014Article
- Expression analysis of an evolutionarily conserved alternative splicing factor, Sfrs10, in age-related macular degeneration.PloS one · 2013Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTransformer (Tra) 2β is a member of the serine/arginine-rich (SR)-like protein family that regulates alternative splicing of numerous genes in a concentration-dependent manner. Several types of cancer cells up-regulate Tra2β expression, while the regulatory mechanism of Tra2β expression remains to be elucidated. In this study, we examined the transcriptional regulation and possible functions of Tra2β in human colon cancer cells.
methodsWe cloned 959 bp-upstream of the human TRA2β 5'-flank into luciferase constructs. Chromatin immunoprecipitation (ChIP) was employed to identify crucial cis element(s) and trans activator(s) of the TRA2β promoter. Tra2β expression in the human colon and colon cancer tissues was examined by immunohistochemistry.
resultsIn response to sodium arsenite, colon cancer cells (HCT116) increased levels of TRA2β1 mRNA encoding a functional, full-length Tra2β with a peak around 6 h without changing its mRNA stability. Transient expression assays using a reporter gene driven by serially truncated TRA2β promoters and Chip assay demonstrated that an Ets1-binding site present at -64 to -55 bp was crucial for basal transcription, while three heat shock elements (HSEs) located at -145 to -99 bp mediated the oxidant-induced transactivation of TRA2β. Tra2β knockdown caused apoptosis of HCT116 cells. Tra2β were preferentially expressed in proliferative compartment of normal human colonic glands and adenocarcinomas, where Ets1 and heat shock factor 1 were also highly expressed.
conclusionsOur results suggest that oxidative stress-responsive Tra2β may play an important role in colon cancer growth.
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