ArticleNano letters2013
Rotational mobility of single molecules affects localization accuracy in super-resolution fluorescence microscopy.
Article in Nano letters, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed.
- SPIFFI enables single-shot super-resolution and multidimensional imaging.Nature methods · 2026Article
- Discriminating single-molecule binding events from diffraction-limited fluorescence.Nature communications · 2025Article
- Resolving the Orientations of and Angular Separation Between a Pair of Dipole Emitters.Physical review letters · 2025Article
- Single-molecule orientation-localization microscopy: Applications and approaches.Quarterly reviews of biophysics · 2024Review
- Combining deep learning approaches and point spread function engineering for simultaneous 3D position and 3D orientation measurements of fluorescent single molecules.Optics communications · 2023Article
- Measurement of Molecular Height Using Cell Surface Optical Profilometry (CSOP).Methods in molecular biology (Clifton, N.J.) · 2023Article
- Photon efficient orientation estimation using polarization modulation in single-molecule localization microscopy.Biomedical optics express · 2022Article
- Resolving the Three-Dimensional Rotational and Translational Dynamics of Single Molecules Using Radially and Azimuthally Polarized Fluorescence.Nano letters · 2022Article
- 4polar-STORM polarized super-resolution imaging of actin filament organization in cells.Nature communications · 2022Article
- Improved localization precision via restricting confined biomolecule stochastic motion in single-molecule localization microscopy.Nanophotonics (Berlin, Germany) · 2022Article
- Single-Molecule Localization Microscopy of 3D Orientation and Anisotropic Wobble Using a Polarized Vortex Point Spread Function.The journal of physical chemistry. B · 2021Article
- Simultaneous orientation and 3D localization microscopy with a Vortex point spread function.Nature communications · 2021Article
- Cryogenic Super-Resolution Fluorescence and Electron Microscopy Correlated at the Nanoscale.Annual review of physical chemistry · 2021Review
- Three-dimensional single molecule localization close to the coverslip: a comparison of methods exploiting supercritical angle fluorescence.Biomedical optics express · 2021Article
- Single-Molecule 3D Orientation Imaging Reveals Nanoscale Compositional Heterogeneity in Lipid Membranes.Angewandte Chemie (International ed. in English) · 2020Article
- Spatio-angular fluorescence microscopy III. Constrained angular diffusion, polarized excitation, and high-NA imaging.Journal of the Optical Society of America. A, Optics, image science, and vision · 2020Article
- Molecular height measurement by cell surface optical profilometry (CSOP).Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Variable incidence angle linear dichroism (VALiD): a technique for unique 3D orientation measurement of fluorescent ensembles.Optics express · 2020Article
- Selective sequestration of signalling proteins in a membraneless organelle reinforces the spatial regulation of asymmetry in Caulobacter crescentus.Nature microbiology · 2020Article
- Molecular resolution imaging by repetitive optical selective exposure.Nature methods · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The asymmetric nature of single-molecule (SM) dipole emission patterns limits the accuracy of position determination in localization-based super-resolution fluorescence microscopy. The degree of mislocalization depends highly on the rotational mobility of SMs; only for SMs rotating within a cone half angle α > 60° can mislocalization errors be bounded to ≤10 nm. Simulations demonstrate how low or high rotational mobility can cause resolution degradation or distortion in super-resolution reconstructions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.