ArticleThe Journal of eukaryotic microbiology
The mitochondrial genome and a 60-kb nuclear DNA segment from Naegleria fowleri, the causative agent of primary amoebic meningoencephalitis.
Article in The Journal of eukaryotic microbiology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Comparative single-cell genomics of two uncultivatedmSphere · 2025Article
- Modes and mechanisms for the inheritance of mitochondria and plastids in pathogenic protists.PLoS pathogens · 2025Review
- Immunogens in Balamuthia mandrillaris: a proteomic exploration.Parasitology research · 2024Article
- Genomics and transcriptomics yields a system-level view of the biology of the pathogen Naegleria fowleri.BMC biology · 2021Article
- Single-cell genomics unveils a canonical origin of the diverse mitochondrial genomes of euglenozoans.BMC biology · 2021Article
- Evolutionary History of Mitochondrial Genomes in Discoba, Including the Extreme Halophile Pleurostomum flabellatum (Heterolobosea).Genome biology and evolution · 2021Article
- Application of the omics sciences to the study of Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris: current status and future projections.Parasite (Paris, France) · 2021Review
- Recent advances in understanding mitochondrial genome diversity.F1000Research · 2020Review
- Horizontally-acquired genetic elements in the mitochondrial genome of a centrohelid Marophrys sp. SRT127.Scientific reports · 2019Article
- The genome of Naegleria lovaniensis, the basis for a comparative approach to unravel pathogenicity factors of the human pathogenic amoeba N. fowleri.BMC genomics · 2018Article
- Mitochondrial Genome Evolution and a Novel RNA Editing System in Deep-Branching Heteroloboseids.Genome biology and evolution · 2017Article
- Group II Intron-Mediated Trans-Splicing in the Gene-Rich Mitochondrial Genome of an Enigmatic Eukaryote, Diphylleia rotans.Genome biology and evolution · 2016Article
- Clinical metagenomic identification of Balamuthia mandrillaris encephalitis and assembly of the draft genome: the continuing case for reference genome sequencing.Genome medicine · 2015Article
- An ensemble strategy that significantly improves de novo assembly of microbial genomes from metagenomic next-generation sequencing data.Nucleic acids research · 2015Article
- Computational identification of putative miRNAs and their target genes in pathogenic amoeba Naegleria fowleri.Bioinformation · 2015Article
- Complex patterns of gene fission in the eukaryotic folate biosynthesis pathway.Genome biology and evolution · 2014Article
- Missing genes, multiple ORFs, and C-to-U type RNA editing in Acrasis kona (Heterolobosea, Excavata) mitochondrial DNA.Genome biology and evolution · 2014Article
- Genome-wide identification of pathogenicity factors of the free-living amoeba Naegleria fowleri.BMC genomics · 2014Article
- Gene content evolution in Discobid mitochondria deduced from the phylogenetic position and complete mitochondrial genome of Tsukubamonas globosa.Genome biology and evolution · 2014Article
- The perils of pathogen discovery: origin of a novel parvovirus-like hybrid genome traced to nucleic acid extraction spin columns.Journal of virology · 2013Article
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Authors and funding
6 authors at 5 institutions in 2 countries.
Funding
Abstract
Naegleria fowleri is a unicellular eukaryote causing primary amoebic meningoencephalitis, a neuropathic disease killing 99% of those infected, usually within 7-14 days. Naegleria fowleri is found globally in regions including the US and Australia. The genome of the related nonpathogenic species Naegleria gruberi has been sequenced, but the genetic basis for N. fowleri pathogenicity is unclear. To generate such insight, we sequenced and assembled the mitochondrial genome and a 60-kb segment of nuclear genome from N. fowleri. The mitochondrial genome is highly similar to its counterpart in N. gruberi in gene complement and organization, while distinct lack of synteny is observed for the nuclear segments. Even in this short (60-kb) segment, we identified examples of potential factors for pathogenesis, including ten novel N. fowleri-specific genes. We also identified a homolog of cathepsin B; proteases proposed to be involved in the pathogenesis of diverse eukaryotic pathogens, including N. fowleri. Finally, we demonstrate a likely case of horizontal gene transfer between N. fowleri and two unrelated amoebae, one of which causes granulomatous amoebic encephalitis. This initial look into the N. fowleri nuclear genome has revealed several examples of potential pathogenesis factors, improving our understanding of a neglected pathogen of increasing global importance.
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