ArticlePLoS neglected tropical diseases2013
A single multilocus sequence typing (MLST) scheme for seven pathogenic Leptospira species.
Article in PLoS neglected tropical diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers.
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115 citing papers in PubMed.
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- Diagnosis of leptospirosis in animals: challenges and perspectives.Journal of clinical microbiology · 2026Review
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- Serological and molecular evidence of Leptospira spp. in martens from Poland.BMC veterinary research · 2026Article
- Comprehensive genotyping and taxonomic analysis uncovers extensive distribution of intermediate Leptospira species in Colombia.World journal of microbiology & biotechnology · 2026Article
- Host-Driven Genetic Diversity ofTransboundary and emerging diseases · 2026Article
- High Resolution Genotyping of Leptospira Direct from Clinical Samples Using Multiplexed Amplicon Sequencing.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Molecular detection of a Leptospira lineage with high similarity to Leptospira kirschneri in human, environmental, and rodent samples from Gilan Province, Iran: an integrated field and laboratory investigation.GMS hygiene and infection control · 2026Article
- A Guidance for Diagnosis, Control Measures, and Surveillance of Leptospirosis in Non-Human Primates From a Veterinary Perspective in a One-Health Approach.Journal of medical primatology · 2025Review
- Shaping the future ofJournal of medical microbiology · 2025Review
- A Retrospective Cohort Study of Leptospirosis in Crete, Greece.Tropical medicine and infectious disease · 2025Article
- Chronic Leptospirosis in a Breeding Bull: A Case Report.Microorganisms · 2025Article
- Host population dynamics influence Leptospira spp. transmission patterns among Rattus norvegicus in Boston, Massachusetts, US.PLoS neglected tropical diseases · 2025Article
- Molecular detection and typing of pathogenicEpidemiology and infection · 2025Article
- Seroprevalence and Molecular Epidemiology ofTransboundary and emerging diseases · 2025Article
- Leptospirosis Risk Assessment in Rodent Populations and Environmental Reservoirs in Humanitarian Aid Settings in Thailand.Microorganisms · 2024Article
- The Challenge of Bacterial Strain Identification:Tropical medicine and infectious disease · 2024Article
55 more citing papers are in PubMed but not listed here.
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21 authors.
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Abstract
backgroundThe available Leptospira multilocus sequence typing (MLST) scheme supported by a MLST website is limited to L. interrogans and L. kirschneri. Our aim was to broaden the utility of this scheme to incorporate a total of seven pathogenic species. METHODOLOGY AND
findingsWe modified the existing scheme by replacing one of the seven MLST loci (fadD was changed to caiB), as the former gene did not appear to be present in some pathogenic species. Comparison of the original and modified schemes using data for L. interrogans and L. kirschneri demonstrated that the discriminatory power of the two schemes was not significantly different. The modified scheme was used to further characterize 325 isolates (L. alexanderi [n = 5], L. borgpetersenii [n = 34], L. interrogans [n = 222], L. kirschneri [n = 29], L. noguchii [n = 9], L. santarosai [n = 10], and L. weilii [n = 16]). Phylogenetic analysis using concatenated sequences of the 7 loci demonstrated that each species corresponded to a discrete clade, and that no strains were misclassified at the species level. Comparison between genotype and serovar was possible for 254 isolates. Of the 31 sequence types (STs) represented by at least two isolates, 18 STs included isolates assigned to two or three different serovars. Conversely, 14 serovars were identified that contained between 2 to 10 different STs. New observations were made on the global phylogeography of Leptospira spp., and the utility of MLST in making associations between human disease and specific maintenance hosts was demonstrated.
conclusionThe new MLST scheme, supported by an updated MLST website, allows the characterization and species assignment of isolates of the seven major pathogenic species associated with leptospirosis.
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