Evidence map›Paper›PMID 23359559›Full record

ArticleNuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms2013

Bomb Pulse Biology.

Miranda J Sarachine Falso, Bruce A Buchholz

Open access · greenAbstract read
In one paragraph

Article in Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Current perspectives on the cellular and molecular features of epigenetic ageing.Experimental biology and medicine (Maywood, N.J.) · 2020
    Review
  3. Article
  4. Article
  5. Review
  6. Operation of the "Small" BioAMS Spectrometers at CAMS: Past and Future Prospects.Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms · 2015
    Article
  7. Article
  8. 14C Analysis of protein extracts from Bacillus spores.Forensic science international · 2014
    Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Miranda J Sarachine FalsoCenter for Accelerator Mass Spectrometry, Mail stop L-397, Lawrence Livermore National Laboratory, P.O. Box 808, Livermore, CA 94551 USA.
Bruce A Buchholz
Lawrence Livermore National Laboratory · US

Funding

VITAMIN C (ASCORBATE) METABOLISM IN DROSPHILA MELANOGASTERP41RR013461 · NCRR · UNIVERSITY OF CALIF-LAWRNC LVRMR NAT LAB · PI ALLEN, LINDSAY H · 1999 to 2011
$20.8M
Resource for the Development of Biomedical Accelerator Mass Spectrometry (AMS)P41GM103483 · NIGMS · UNIVERSITY OF CALIF-LAWRNC LVRMR NAT LAB · PI TURTELTAUB, KENNETH W. · 2012 to 2019
$12.0M
Quantitation of protein turnover in the human adult lens using the 14C bomb-pulseR21EY018722 · NEI · UNIVERSITY OF CALIF-LAWRNC LVRMR NAT LAB · PI BUCHHOLZ, BRUCE A · 2008 to 2009
$522k
NCRR NIH HHS P41 RR013461NEI NIH HHS R21 EY018722NIGMS NIH HHS P41 GM103483
6 · The paper itself

Abstract

The past decade has seen an explosion in use of the (14)C bomb-pulse to do fundamental cell biology. Studies in the 1960's used decay counting to measure tissue turnover when the atmospheric (14)C/C concentration was changing rapidly. Today bulk tissue measurements are of marginal interest since most of the carbon in the tissue resides in proteins, lipids and carbohydrates that turn over rapidly. Specific cell types with specialized functions are the focus of cell turnover investigations. Tissue samples need to be fresh or frozen. Fixed or preserved samples contain petroleum-derived carbon that has not been successfully removed. Cell or nuclear surface markers are used to sort specific cell types, typically by fluorescence-activated cell sorting (FACS). Specific biomolecules need to be isolated with high purity and accelerator mass spectrometry (AMS) measurements must accommodate samples that generally contain less than 40 micrograms of carbon. Furthermore, all separations must not add carbon to the sample. Independent means such as UV absorbance must be used to confirm molecule purity. Approaches for separating specific proteins and DNA and combating contamination of undesired molecules are described.

Identifiers

PMID23359559
PMCPMC3555406
OpenAlexW2074425996

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.