ArticlePloS one2013
The small GTPase Rif is dispensable for platelet filopodia generation in mice.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Roles of G proteins and their GTPase-activating proteins in platelets.Bioscience reports · 2024Review
- Transcriptional profile of platelets and iPSC-derived megakaryocytes from whole-genome and RNA sequencing.Blood · 2021Article
- High Expression of RhoF Predicts Worse Overall Survival: A Potential Therapeutic Target for non-M3 Acute Myeloid Leukemia.Journal of Cancer · 2021Article
- Fast-cycling Rho GTPases.Small GTPases · 2020Review
- Characterisation of the Ral GTPase inhibitor RBC8 in human and mouse platelets.Cellular signalling · 2019Article
- Building Blood Vessels-One Rho GTPase at a Time.Cells · 2019Review
- Article
- Review
- KLF4 activates NFκB signaling and esophageal epithelial inflammation via the Rho-related GTP-binding protein RHOF.PloS one · 2019Article
- Leukemia-associated Rho guanine-nucleotide exchange factor is not critical for RhoA regulation, yet is important for platelet activation and thrombosis in mice.Journal of thrombosis and haemostasis : JTH · 2015Article
- Platelet actin nodules are podosome-like structures dependent on Wiskott-Aldrich syndrome protein and ARP2/3 complex.Nature communications · 2015Article
- Platelet Rho GTPases-a focus on novel players, roles and relationships.The Biochemical journal · 2015Review
- Syntaxin 8 regulates platelet dense granule secretion, aggregation, and thrombus stability.The Journal of biological chemistry · 2015Article
- Massive localized malignant pleural mesothelioma (LMPM): manifestations on computed tomography in 6 cases.International journal of clinical and experimental medicine · 2015Article
- Rhof promotes murine marginal zone B cell development.Nagoya journal of medical science · 2014Article
- RhoG protein regulates platelet granule secretion and thrombus formation in mice.The Journal of biological chemistry · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
backgroundFormation of filopodia and other shape change events are vital for platelet hemostatic function. The mechanisms regulating filopodia formation by platelets are incompletely understood however. In particular the small GTPase responsible for initiating filopodia formation by platelets remains elusive. The canonical pathway involving Cdc42 is not essential for filopodia formation in mouse platelets. The small GTPase Rif (RhoF) provides an alternative route to filopodia generation in other cell types and is expressed in both human and mouse platelets. HYPOTHESIS/
objectiveWe hypothesized that Rif might be responsible for generating filopodia by platelets and generated a novel knockout mouse model to investigate the functional role of Rif in platelets. METHODOLOGY/PRINCIPAL
findingsConstitutive RhoF(-/-) mice are viable and have normal platelet, leukocyte and erythrocyte counts and indices. RhoF(-/-) platelets form filopodia and spread normally on various agonist surfaces in static conditions and under arterial shear. In addition, RhoF(-/-) platelets have normal actin dynamics, are able to activate and aggregate normally and secrete from alpha and dense granules in response to collagen related peptide and thrombin stimulation.
conclusionsThe small GTPase Rif does not appear to be critical for platelet function in mice. Functional overlap between Rif and other small GTPases may be responsible for the non-essential role of Rif in platelets.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.